Summary
By using Ca2+-sensitive fluorescent probe, Fura-2, the effects of endothelial cell-conditioned medium and hypoxia on intraceilular free calcium ([Ca2+]) in cultured pulmonary artery smooth muscle cell (PASMC) were studied. Normoxic porcine pulmonary artery endothelial cell-conditioned medium (NPAECCM) obviously elevated [Ca2+]i in PASMC, whereas the hypoxic porcine pulmonary artery endothelial cell conditioned medium (HPAECCM) significantly elevated [Ca2+]i in PASMC much more than NPAECCM. Both the effects of NPAECCM and HPAEC-CM were dependent on the cultured endothelial cell extracellular calcium concentrations, ranged from 1. 8 mmol/L to 2.4 mmol/L. Meanwhile, hypoxia directly increased, which was partially inhibited by verapamil, [Ca2+]i in PASMC through Ca2+ influx pathway.
The data suggest that the augmented regulation of endothelial cell on PASMC via Ca2+ second messenger system and the hypoxia-induced Ca2+ influx into PASMC, particularly the former, may be components of mechanisms underlying hypoxic pulmonary vasoconstriction and chronic pulmonary hypertension.
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This study was supported by grant from Eighth Five-Year Plan of Science and Technology of China
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Qing-hua, H., Di-xun, W. Both hypoxic endothelial cell conditioned medium and hypoxia elevate intracellular free calcium in pulmonary artery smooth muscle cells. Journal of Tongji Medical University 14, 200–203 (1994). https://doi.org/10.1007/BF02897667
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DOI: https://doi.org/10.1007/BF02897667