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Rat DARPP-32: Cloning, sequencing, and characterization of the cDNA

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Abstract

DARPP-32, a substrate for cyclic AMP-dependent protein kinase, is highly enriched in the caudate nucleus. In the present study, the cDNA for rat DARPP-32 was isolated and characterized. When compared to the coding region of bovine DARPP-32 cDNA, there was 86% identity at the nucleotide level, and 84% identity at the amino acid level. The homology in the region previously noted to be similar to phosphatase inhibitor-I remained intact. There were also 3′-untranslated regions that were highly conserved. The DARPP-32 mRNA, which appeared as a doublet, was widely distributed in the central nervous system and was highly enriched in the striatum. Southern blot analysis revealed a simple hybridization pattern, consistent with the presence of a single gene coding for rat DARPP-32. Unilateral destruction of the nigrostriatal dopaminergic pathway by injection of 6-hydroxydopamine (6-OHDA) into the substantia nigra did not alter the striatal steady state levels of DARPP-32 mRNA.

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Author notes

  1. Tatsuya Kurihara

    Present address: Suntory Institute for Biomedical Research, Osaka, Japan

Authors and Affiliations

  1. Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, 1230 York Avenue, 10021, New York, NY, USA

    Michelle E. Ehrlich, Tatsuya Kurihara & Paul Greengard

Authors
  1. Michelle E. Ehrlich
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  2. Tatsuya Kurihara
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Additional information

This work was supported by Physician Scientist Award MH00606 (to M.E.E.) and MH40899 (to P.G.) from the National Institute of Mental Health.

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Ehrlich, M.E., Kurihara, T. & Greengard, P. Rat DARPP-32: Cloning, sequencing, and characterization of the cDNA. J Mol Neurosci 2, 1–10 (1990). https://doi.org/10.1007/BF02896920

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