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Effect of ethionine on the synthesis of β-galactosidase inEscherichia coli

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Abstract

Ethionine at concentrations of 10−3M, 5×10−3M and 10−2M inhibits growth, both of β-galactosidase inducible ML-30 and constitutive ML-308Escherichia coli strains. The protein synthesis (measured by the incorporation of l-leucine-14C and l-aspartic-14C acid into proteins) of these strains is inhibited to the same extent as their growth. The synthesis of inducible and constitutive β-galactosidase produced by the strains ML-30 and ML-308, respectively, is considerably inhibited by ethionine.

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References

  • Bernheim, F.:Rapid reversal of ethionine inhibition of enzyme induction in Pseudomonas aeruginosa by l- and d- methionine. Proc. Soc. exptl. Biol. Med. 101: 346, 1959.

    CAS  Google Scholar 

  • Cohn, M., Monod, J.:Purification et propriétés de la β-galactosidase (lactase) d'Escherichia coli. Biochim. biophys. Acta 7: 153, 1951.

    Article  PubMed  CAS  Google Scholar 

  • Farber, E.:Ethionine carcinogenesis. Advan. Cancer Res. 7: 383, 1963.

    Article  CAS  Google Scholar 

  • Fischer, J. F., Mallette, M. F.:The natural occurrence of ethionine in bacteria. J. gen. Physiol. 45: 1, 1961.

    Article  Google Scholar 

  • Gross, D., Tarver, H.:Studies on ethionine. IV. The incorporation of ethionine into the proteins of Tetrahymena. J. biol. Chem. 217: 169, 1955.

    PubMed  CAS  Google Scholar 

  • Halvorson, H. O., Spiegelman, S.:The inhibition of enzyme formation by amino acid analogues. J. Bacteriol. 64: 207, 1952.

    Article  PubMed  CAS  Google Scholar 

  • Lowry, O. H., Rosenbrough, M. J., Farr, A. L., Randall, R. J.:Protein measurement with the Folin phenol reagent. J. biol. Chem. 193: 265, 1951.

    PubMed  CAS  Google Scholar 

  • Lupu, C. I., Farber, E.:Effects of ethionine upon hepatic glycogen formation from glucose in intact rats. Proc. Soc. exptl. Biol. Med. 86: 701, 1954.

    CAS  Google Scholar 

  • Maw, G. A.:Ability of S-methyl-l-cysteine to annul the inhibition of yeast growth by l-ethionine and by S-ethyl-l-cysteine. J. gen. Microbiol. 25: 44, 1961.

    Google Scholar 

  • Maw, G. A.:Entry of ethionine sulphur into yeast protein. Biochem. J. 98: 280, 1966.

    Google Scholar 

  • Parks, L. W.:S-adenosylethionine and ethionine inhibition. J. biol. Chem. 232: 169, 1958.

    PubMed  CAS  Google Scholar 

  • Rickenberg, H. V., Yanofski, C., Bonner, D. M.:Enzymatic deadaptation. J. Bacteriol. 66: 683, 1953.

    PubMed  CAS  Google Scholar 

  • Shull, K. H.:Hepatic phosphorylase and adenosine triphosphate levels in ethionine-treated rats. J. biol. Chem. 237: PC 1734, 1962.

    PubMed  CAS  Google Scholar 

  • Simpson, M. V., Farber, E., Tarver, H.:Studies on ethionine. I. Inhibition of protein synthesis in intact animals. J. biol. Chem. 182: 81, 1950.

    CAS  Google Scholar 

  • Smith, R. C., Salmon, W. D.:Effect of ethionine on the ribonucleic acid, deoxyribonucleic acid and protein content of Escherichia coli. J. Bacteriol. 89: 687, 1965a.

    PubMed  CAS  Google Scholar 

  • Smith, R. C., Salmon, W. D.:Enhancement of the inhibition of Salmonella typhimurium by ethionine. J. Bacteriol. 89: 1494, 1965b.

    PubMed  CAS  Google Scholar 

  • Stekol, J. A.:Biochemical basis for ethionine effects on tissues. Advan. Enzymol. 25: 369, 1963.

    CAS  Google Scholar 

  • Villa-Trevino, S., Farber, E.:The reversal by adenosine triphosphate of ethionine-induced inhibition of protein synthesis. Biochim. biophys. Acta 61: 649, 1962.

    CAS  Google Scholar 

  • Villa-Trevino, S., Shull, K. H., Farber, E.:The role of adenosine triphosphate deficiency of ethionine-induced inhibition of protein synthesis. J. biol. Chem. 238: 1757, 1963.

    PubMed  CAS  Google Scholar 

  • Wainwright, S. D.:On the development of increased tryptophan synthetase enzyme activity by cell-free extracts of Neurospora crassa. Can. J. Biochem. Physiol. 37: 1417, 1959.

    CAS  Google Scholar 

  • Yoshida, A.:Mechanism of protein synthesis: bacterial α-amylase containing ethionine. Biochem. biophys. Acta 29: 213, 1958.

    Article  PubMed  CAS  Google Scholar 

  • Yoshida, A., Yamasaki, M.:Studies on the mechanism of protein synthesis. Incorporation of ethionine into α-amylase of Bacillus subtilis. Biochim. biophys. Acta 34: 158, 1959.

    Article  PubMed  CAS  Google Scholar 

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Spížek, J., Janeček, J. Effect of ethionine on the synthesis of β-galactosidase inEscherichia coli . Folia Microbiol 12, 140–145 (1967). https://doi.org/10.1007/BF02896874

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