Abstract
Suspension cultures of tomato cells were used to study the membrane injury by the toxic concentration of mercuric chloride. Assessment of electrolyte leakage, UV-absorbance of the tissue leachates, relative leakage ratio, injury index, membrane lipid peroxidation, lipoxygenase activity, α-amino nitrogen and total soluble carbohydrate contents showed the extent of membrane damage as a function of the increasing concentration of mercuric chloride. It is suggested that the selected parameters can be used as qualitative tests for determination of stress-induced membrane damage
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Abbreviations
- LOX:
-
lipoxygenase
- PUFA:
-
poly-unsaturated fatty acids
- MDA:
-
malon dialdehyde
- EC:
-
electric conductivity
- 2,4-D:
-
2,4-dichlorophenoxy acetic acid
- RLR:
-
relative leakage ratio
- α-NH2 :
-
α-amino nitrogen
- TBA:
-
thiobarbituric acid
- d.m.:
-
dry mass.
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De, B., Mukherjee, A.K. Mercuric chloride induced membrane damage in tomato cultured cells. Biol Plant 38, 469–473 (1996). https://doi.org/10.1007/BF02896684
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DOI: https://doi.org/10.1007/BF02896684