Skip to main content
SpringerLink
Log in

Cloning and sequencing of cDNA fragment of gene of wheat (Triticum aestivum. L) induced by dehydration

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

cDNA fragment of the gene (dehydration induced,di1) of wheat (Triticum aestivum. L) induced by 30% PEG-6000 (−1.13 MPa) treatment was isolated with mRNA differential display technique. Northern blot analysis showed that the expression ofdi1 gene improved at 10 h reached the highest at 48 h under 30% PEG-6000 treatment. cDNA fragment ofdi1 gene has been cloned and sequenced (211 bp). DNA sequence analysis shows that there is no homologue in GenBank todi1 cDNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhu, J. K., Hasegawa, P. M., Bressan, R. A., Molecular aspects of osmotic stress in plants,Curr. Rev. in Plant Sci., 1997, 3: 253.

    Article  Google Scholar 

  2. Ingram, J., Bartels, D., The molecular basis of dehydration tolerance in plants,Annu. Rev. Plant Physiol. Plant Mol. Biol., 1996, 47: 377.

    Article  Google Scholar 

  3. Liang, P., Pardee, A. B., Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction,Science, 1992, 257: 967.

    Article  Google Scholar 

  4. Qiu, Q. S., Li, L., Liang, H. G.et al., Effect of water stress on the Redox system of the plasma membrane of wheat Roots,Acta Phytophysiologicu Sinica (in Chinese), 1994, 1: 145.

    Google Scholar 

  5. Chomczynski, P., Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,Analytical Biochemistry, 1987, 162: 156.

    Article  Google Scholar 

  6. Sambrook, J., Fritsh, E. F., Maniatis, T.,Molecular Cloning: A Laboratory Manual, 2nd ed., New York: Cold Spring Harbor Laboratory Press, 1989.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 200032, Shanghai, China

    Jianming Zhou, Yongyan Bai & Zhangcheng Tang

  2. Salk Institute for Biological Studies, 92037, CA, USA

    Qun Zhu

Authors
  1. Jianming Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qun Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yongyan Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhangcheng Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Zhou, J., Zhu, Q., Bai, Y. et al. Cloning and sequencing of cDNA fragment of gene of wheat (Triticum aestivum. L) induced by dehydration. Chin.Sci.Bull. 44, 230–233 (1999). https://doi.org/10.1007/BF02896280

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02896280

Keywords

Search

Navigation