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Effect of PD I administration on dopamine receptors mRNAs expression in the lesioned striatum of PD rat model

Journal of Huazhong University of Science and Technology [Medical Sciences] volume 25, pages 645–647 (2005)Cite this article

Summary

To study the effect of PD I administration on dopamine receptors (DR1, DR2) mRNAs expression in the lesioned striatum of the PD rat model and confirm if PD I has the effect of dopamine receptor agonist. The PD rats with unilateral 6-hydroxydopamine lesioned were administrated with PD I, L-dopa methyl/benserazide, L-dopa methyl/benserazide/PD I, normal saline respectively for 4 weeks and their behavioral changes were observed. Then the rats were sacrificed and RT-PCR technique was used to detect changes of dopamine receptors (DR1, DR2) mRNAs expression in the ipsilateral striatum 1 day after the last treatment. The results showed that treatment with PD I plus L-dopa resulted in a stable contralateral rotation behavior; treatment with L-dopa resulted in a progressively increased contralateral rotation behavior. Rotation behavior induced by anhydromorphine decreased with PD I or PD I plus L-dopa treatment. Treatment With L-dopa or PD I plus L-dopa, up-regulation of DR1 mRNA and down-regulation of DR2 mRNA were observed in the ipsilateral striatum which were more obvious than that treated with PD I or vehicle (P<0.05). It was concluded that long-term treatment with PD I could alleviate the behavior of PD rats. PD I had no apparent effect on the dopamine receptors (DR1, DR2) mRNAs expression in the ipsilateral striatum and the PD I has no agonist effect on dopamine receptors.

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Affiliations

  1. Department of Neurology, Union Hospital, Tongji Medical College, Huzhong University of Science and Technology, 430022, Wuhan, China

    Yang Mei, Sun Shenggang & Cao Xuebing

Authors
  1. Yang Mei
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  2. Sun Shenggang
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  3. Cao Xuebing
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Additional information

YANG Mei, female, born in 1975, M. D., Ph. D.

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Mei, Y., Shenggang, S. & Xuebing, C. Effect of PD I administration on dopamine receptors mRNAs expression in the lesioned striatum of PD rat model. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 25, 645–647 (2005). https://doi.org/10.1007/BF02896159

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  • DOI: https://doi.org/10.1007/BF02896159

Key words

  • Parkinson's disease
  • 6-hydroxydopamine
  • dopamine receptor