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Changes of 3,4-dihydroxybenzoic and 4-hydroxybenzoic acids inNicotiana tabacum cell suspension culture

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Biologia Plantarum

Abstract

The optimum conditions for cell suspension culture experiments have been characterized by respiration,p-Phenylenediamine oxidase and growth of cell suspension ofNicotiana tabacum for 15 days in stationary culture. Change of 3,4-Dihydroxybenzoic and 4-Hydroxybenzoic acids both present at 10−4 M in culture medium were studied in short term experiments. An oxidation product of 3,4-Dihydroxybenzoic acid characterized by an absorption maximum at 280 nm was isolated after 1 h of incubation. The 4-Hydroxybenzoic acid did not change when incubated for 4 h under the same conditions.

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References

  • Bartoš, J., Berková, E., Šetlík, I.: A versatile chamber for gas exchange measurements in suspensions of algae and chloroplasts. - Photosynthetica9: 395–406, 1975.

    Google Scholar 

  • Berlin, J., Barz, W.: Oxidative decarboxylation of para-hydroxybenzoic acids by peroxidases underin vivo andin vitro conditions. - Z. Naturforsch.30c: 650–658, 1975a.

    CAS  Google Scholar 

  • Berlin, J., Barz, W.: Stoffwechsel von Benzoesäuren in Pflanzlichen Zellsuspensionskulturen.-Planta med. (PLME Supplement): 134–139, 1975b.

  • Fuchs, A.: Polyphenol oxidases and phenolics in relation to resistance against cucumber scab inCucumis sativus. I. Fungal and host polyphenol exidases. - Meth. J. Plant Pathol.71: 157 to 166, 1965.

    Google Scholar 

  • Harms, H.: Metabolism of soil-related compounds in plants and cell suspension cultures. - International Symposium Studies on Soil Organic Matter. - FAO, Brunswick 1976.

    Google Scholar 

  • Harms, H., Haider, K., Berlin, J., Kiss, P., Barz, W.: Über O-Demethylierung und Decarboxylierung von Benzoesäuren in pflanzlichen Zellsuspensionskulturen. - Planta105: 342 to 351, 1972.

    Article  Google Scholar 

  • Pierpoint, W. S.: The enzymic oxidation of chlorogenic acid and some reactions of the quinone produced. - Biochem. J.98: 567–580, 1966.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1, 160 00, Praha 6, Czechoslovakia

    Marie Hrubcová, Milena Cvikrová & F. Pospíšil

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  1. Marie Hrubcová
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  2. Milena Cvikrová
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  3. F. Pospíšil
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Hrubcová, M., Cvikrová, M. & Pospíšil, F. Changes of 3,4-dihydroxybenzoic and 4-hydroxybenzoic acids inNicotiana tabacum cell suspension culture. Biol Plant 23, 318–320 (1981). https://doi.org/10.1007/BF02895380

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  • DOI: https://doi.org/10.1007/BF02895380

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