Abstract
FUdR 10−4 M was applied together with [3H] TdR on the growing barley embryos cultivated in the nutrient solution. Samples were fixed 1–4 h after the onset of the treatment and microautoradiograms were prepared. All the mitoses were unlabelled, while more than 150 autoradiographic grains were found above labelled interphase nuclei; no cells that terminated S phase at the onset of the treatment passed through entire G2 and reached mitosis during the 4 h duration of the treatment. Chromosomal fragments in anaphases appeared the first hour after the onset of the treatment. Their frequency increased from the first to the second hour and remained almost constant from the second to the fourth hour. FUdR induces chromosomal fragments in barley root meristems also in G2 phase of the mitotic cycle.
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Abbreviations
- FUdR:
-
fluorodeoxyuridine
- TdR:
-
thymidine
- MI:
-
mitotic index
- LI:
-
labelling index,i.e. the per cent proportion of cell nuclei labelled by autoradiographic grains.
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Ondřej, M., Schwammenhöferová, K. Autoradiographic study of the effect of 5-Fluorodeoxyuridine on barley chromosomes. Biol Plant 23, 261–265 (1981). https://doi.org/10.1007/BF02895361
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DOI: https://doi.org/10.1007/BF02895361