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Two-dimensional crystallization and preliminary structure analysis of LHC-II from cucumber and spinach

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Abstract

Large and well-ordered two-dimensional (2D) crystals of the light-harvesting chlorophyll a/b protein complexes (LHC-II) from cucumber and spinach chloroplasts were produced by the so-called batch method. The twodimensional structures of these crystals were examined at about 1.5 nm resolution by electron microscopy and image processing. The projection maps showed that there were similar, but not identical, structure features between two different LHC-II complexes. A cmparison between 2D crystal formations of the two different LHC-II complexes was done and some factors affecting 2D crystallization of the membrane proteins were analyzed. The relations of the structures of the LHC-II complexes to their polypeptide components and Chl a/b ratio were also discussed.

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References

  1. Kühlbrandt, W., Two-dimensional crystallization of membrane proteins,Quarterly Reviews of Bioplaysics, 1992, 25: 1.

    Article  Google Scholar 

  2. Amos, L. A., Henderson, R., Unwin, P. N. T., Three-dimensional structure determination by electron microscopy of two-dimensional crystals,Prog. Biophys. Molec. Biol., 1982, 39: 183.

    Article  CAS  Google Scholar 

  3. Henderson, R., Baldwin, J. M., Ceska, T. A. et al., Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy,J. Mol. Biol., 1990, 213: 899.

    Article  PubMed  CAS  Google Scholar 

  4. Li, J., Light-harvesting chlorophyll a/b-protein: three-dimensional structure of a reconstituted membrance lattice in negatively stain,Proc. Sci. USA, 1985, 82: 386.

    Article  CAS  Google Scholar 

  5. Lyon, M. K., Unwin, P. N. T., Two-dimensional structure of the light-harvesting chlorophyll a/b complex by cryoelectron microscopy,J. Cell Biol., 1988, 106: 1515.

    Article  PubMed  CAS  Google Scholar 

  6. Kühlbrandt, W., Toler, T., Wehrli, E., The structure of membrane crystals of the light-harvesting chlorophyll a/b protein complex.J. Cell. Biol., 1983, 96: 1414.

    Article  PubMed  Google Scholar 

  7. Kühlbrandt, W., Wang, D. N., Fujiyoshi, Y., Atomic model of plant light-harvesting complex by elactron crystallography.Nature, 1994, 367: 614.

    Article  PubMed  Google Scholar 

  8. Wang, D. N., Kühlbrandi, W., High-resolution electron crystallography of light-harvesting chlorophyll a/b-protein complex in three different media,j. Mol. Biol., 1991, 217: 691.

    Article  PubMed  CAS  Google Scholar 

  9. Lou Shiqing, Wang Kebin, Kuang Tingyun et al., A comparative study on PS II light-harvesting chlorophyll a/b protein complexes between spinach and cucomber,Acta Rotanica Sinica (in Chinese), 1995, 37: 192.

    Google Scholar 

  10. Michel, H., Griffin, P. R., Shabanowith, J. et el., Tandem mass spectrometry identifies sites of three post-translational modifications of spinach light-harvesting chlorophyll protein II,J. Biol. Chem., 1991, 266: 17584.

    PubMed  CAS  Google Scholar 

  11. Xu Chunhui, Zhao Fuhong, Wang Kebin et al., Isolation of light-harvesting chlorophyll a/b protein complex and its spectral characteristics,Botanical Research, 1993, (7): 198.

  12. Nuβberger, S., Dörr, K., Wang, D. N., Lipid-protein interactions in crystal of plant light-harvesting complex,J. Mol. Biol., 1993, 234: 347.

    Article  Google Scholar 

  13. Kühlbrandt, W., Three-dimensional structure of the light-harvesting chlorophyll a/b-protein complex.Nature, 1984. 307: 478.

    Article  Google Scholar 

  14. Kühlbrandt, W., Wang, D. N., Three-dimensional structure of light-harvesting complex determined by electron crystallography.Nature, 1991, 350: 130.

    Article  PubMed  Google Scholar 

  15. Unwin, P. N. T., Ennis, P. D., Two configurations of a channel-forming membrane protein,Nature, 1984, 307: 609.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China

    Wei Xu & Youguo Huang

  2. Beijing Laboratory of Electron Microscopy, Center for Condensed Matter Physics, Chinese Academy of Sciences, 100080, Beijing, China

    Wei Xu & Xing Zhang

  3. Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    Shiqing Lou, Kebin Wang & Tingyun Kuang

Authors
  1. Wei Xu
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  2. Xing Zhang
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  3. Shiqing Lou
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  4. Kebin Wang
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  5. Youguo Huang
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  6. Tingyun Kuang
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Additional information

Project supported by the National Natural Science Foundation of China (Grant No.39370165).

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Xu, W., Zhang, X., Lou, S. et al. Two-dimensional crystallization and preliminary structure analysis of LHC-II from cucumber and spinach. Sci. China Ser. C.-Life Sci. 41, 265–271 (1998). https://doi.org/10.1007/BF02895101

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  • DOI: https://doi.org/10.1007/BF02895101

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