Abstract
Yolk DNA was detected with immunocytochemistry in fertilized chicken eggs. Yolk spheres were purifield with Ficoll-400 density gradient centrifugation, followed by extraction of DNA therein. Yolk DNA is sensitive to various restriction endonucleases. but shows no obvious bands, demonstrating its sequence heterogeneity. There is only a little, if any, methylated CpG in yolk DNA. judgmg from MspI and HpII digestion. Southern blot indicated that the sequence of yolk DNA accounts for a small portion of that of genomic DNA. Its uniqueness suggests that yolk DNA may play a crucial role in early development of chickens.
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Project supported by the National Natural Science Foundation of China (Grant No. 39670361).
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Sun, L., Yu, L. & Chen, C. Presence, isolation and characterization of yolk DNA from chicken eggs. Sci. China Ser. C.-Life Sci. 41, 251–257 (1998). https://doi.org/10.1007/BF02895099
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DOI: https://doi.org/10.1007/BF02895099