Abstract
The effects of G protein and cGMP on phytochrome-mediated amaranthin biosynthesis inAmaranthus caudatus seedlings were studied. It was shown that G protein agonist cholera toxin induced amarathin synthesis in darkness, whereas G protein antagonist pertussis toxin inhibited red light-induced amaranthin synthesis. Amaranthin synthesis was also induced by exogenous cGMP, while the amaranthin biosynthesis induced by cholera toxin, red light and exogenous cGMP was inhibited by genistein. L Y-83583, an inhibitor of guanylyl cyclase, inhibited the amarenthin synthesis induced both by red light and cholera toxin, while it was not able to inhibit the amaranthin synthesis induced by exogenous cGMP. These results suggest that G protein, guanylyl cyclase and cGMP were the candidates in phytochrone signal transduction chain for red light-induced amaranthin biosynthesis and the red light signal transduction chain might be as follows: red light → phytochrome → G protein → guanylyl cyclase → cGMP.
Similar content being viewed by others
References
Ma, L. G., Sun, D. Y., The progress of red light signal transduction in plant cells,Chinese J. Cell Biology (in Chinese), 1996, 18(3): 115.
Quail, P. H., Photosensory perception and signal transduction in plant,Current Opinion in Genetics and Development, 1994, 4: 652.
Quail, P. H., Boylan, M. T., Parks, B. M. et al., Phytochrome: Photosensory perception and signal transduction,Science, 1995, 268: 753.
Shacklock, P. S., Read, N. D., Trewavas, A. J., Cytosolic free calcium mediated red light-induced photomorphogenesis,Nature. 1992, 358: 753.
Romero, L. C., Lam, E., Guanine nucleotide-binding protein involvement in early steps of phytochrome-regulated gene expression,Proc. Natl. Acad. Sci. USA, 1993, 90: 1465.
Neuhaus, G., Bowler, C., Kern, R. et al., Calcium/calmodulin-dependent and independent phytochrome signal transduction pathways.Cell, 1993, 73: 937.
Raghuram, N., Sopory, S., Evidence of some common signal transduction events for opposite regulation of nitriate redutase and phytochrome gene expression by light,Plant Molecular Biology. 1995, 29: 25.
Ma, L. G., Zhou, J. L., An, Y. et al., Effect of calcium on phytochrome-mediated synthesis of amaranthin in Amaranthus caudotus seedlings.Chinese Science Bulletin (in Chinese), 1995, 40: 944.
Bowler, C., Neuhaus, G., Yamagata, H. et al., Cyclic GMP and calcium mediate phytochrome phototransduction,Cell. 1994, 77: 73.
Tong, Z., Liang, B. E., Photomodulated synthesisof amaranthin inAmaranthus caudatus, Acta Phytophysiological Sinica (in Chinese), 1989, 15: 1.
Bowler, C., Yamagastsa, H., Neuhaus, G. et al., Phytmhrome signal transduction pathways are regulatd by reciprocal mechanism,Genes Development, 1994, 8: 2188.
Ma, H., GTP-binding proteins in plants: new members of an old family.Plant Molecular Biology, 1994, 26: 1611.
Warpeha, K. M. F., Hamm, H. E., Rasenick, M. M. et al., A blue-light-activated GTP-binding protein in the plasma membranes of etiolated peas,Prac. Nutl. Acad. Sci. USA, 1991, 88: 8925.
Puge, E. N., Lisman, J., Payne, R., Strange case of the third eye,Nature, 1993, 364: 3889.
Darcy, P. K., Wilczynskas, Z., Fisher, P. R., The role of cGMP in photosensory and thermosensory transduction inDictyostelium discoideum, Microbiology. 1994, 140: 1619.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zhou, J., Ma, L. & Sun, D. Effects of G protein and cGMP on phytochrome-mediated amaranthin synthesis inAmaranthus caudatus seedlings. Sci. China Ser. C.-Life Sci. 41, 232–237 (1998). https://doi.org/10.1007/BF02895096
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02895096