Abstract
Specifically labelled14C-d-glucose was used to estimate the percentage participation of glycolysis and pentose phosphate cycle in the glucose catabolism ofCandida utilis andSaccharomyces cerevisiae. The two yeasts were cultivated at various growth rates (0.1 to 0.5 h−1) in a chemostat on synthetic medium limited with glucose under aerobic conditions. The results show a considerable increase in the percentage participation of pentose phosphate cycle in the glucose catabolized bySaccharomyces cerevisiae with the increase in specific growth rate. However, inCandida utilis, the specific growth rate does not influence significantly the part of glucose catabolized via pentose phosphate cycle, but its absolute values are relatively higher than inSaccharomyces cerevisiae. A rough quantitative estimate indicates that a maximum of 60 to 72% of the assimilated glucose is catabolized through the pentose phosphate cycle while inSaccharomyces cerevisiae the percentage participation of the pentose phosphate cycle varies from 24 to 60% (maximum) and 9 to 34% (minimum).
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Beck C., von Meyenburg H. K.: Enzyme pattern and aerobic growth ofSaccharomyces cerevisiae under various degrees of glucose limitation.J. Bacteriol. 96, 479 (1968).
Beran K.: Continuous flow cultivation of baker’s yeast on beet molasses wort. p. 122 In:Continuous Cultivation of Microorganisms. A symposium, Publ. House Czechoslov. Acad. Sci., Prague, 1958.
Beran K., Zemanová J.: Cell growth and population activity ofSaccharomyces cerevisiae in two-stage continuous cultivation.Biotechnol. Bioeng. 11, 853 (1969).
Brady R. J., Chambliss G. H.: The lack of phosphofructokinase activity in several species ofRhodotorula.Biochem. Biophys. Res. Commun. 29, 898 (1967).
Chakravorty M., Veiga L. A., Bacila M., Horecker B. L.: Pentose metabolism inCandida. II. The diphosphopyridine nucleotide-specific polyol dehydrogenase ofCandida utilis.J. Biol. Chem. 237, 1014 (1962).
David S., Renault J.: Sur la biosynthese du ribose des acides nucleiques deTorula utilis.Compt. Rend. 239, 369 (1954).
De Deken H.: The Crabtree effect. A regulatory system in yeast.J. Gen. Microbiol. 44, 149 (1965).
Fiechter A., Mian F. A., Ris H., Halvorson H. O.: Characterisation of insoluble protein fractions of mitochondria fromSaccharomyces cerevisiae.J. Bacteriol. 109, 855 (1972).
Höfer M.: Estimation of pathways of glucose catabolism inRhodotorula gracilis.Fol. Microbiol. 13, 373 (1968).
Horecker B. L., Rosen O. M., Kowal J., Rosen S., Scher B., Lai C. Y., Hoffee P., Cremona T.: Comparative study of aldolases and diphosphatases, p. 71. In: A. K. Millis (Ed.):Aspects of Yeast Metabolism. Blackwell Scientific Publications, Oxford and Edinburgh, 1968.
Mian F. A., Fencl Z., Prokop A.: Growth rate and enzymes activity in yeast (Candida utilis). p. 105, In: I. Málek, K. Beran, Z. Fencl, V. Munk, J. Řičica and H. Smrčková (Eds.):Continuous Cultivation of Microorganisms, 4th Internat. symp. held in Prague, June 17–21, 1968, Academia, Prague 1969.
Mian F. A., Küenzi M. T., Halvorson H. O.: Studies on mitochondrial membrane proteins inSaccharomyces cerevisiae under different degrees of glucose repression.J. Bacteriol. 115, 976 (1973).
Mian F. A., Prokop A., Fencl Z.: Growth and physiology of yeast cultivated in batch and continous culture systems.Folia Microbiol. 16, 249 (1971).
Olson B. H., Johnson M. J.: Factors producing high yeast yield in synthetic media.J. Bacteriol. 57, 235 (1949).
Sowden J. C., Frankel S., Moor B. H., McClary J. E.: Utilization of 1-14C-d-glucose byTorula utilis yeast.J. Biol. Chem. 206, 547 (1954).
Swanson W. H., Clifton C. E.: Growth and assimilation in cultures ofSaccharomyces cerevisiae.J. Bacteriol. 56, 115 (1948).
Tempest D. W., Herbert D.: Effect of dilution rate and growth limiting substrate on the metabolic activity ofTorula utilis culture.J. Gen. Microbiol. 41, 143 (1965).
Umbreit W. W., Burris R. H., Stauffer J. F.: Manometric Techniques. Burgess Publishing Co., Minneapolis 1957.
Wang C. H., Krackov J. K.: The catabolic fate of glucose inBacillus subtilis.J. Biol. Chem. 237, 3614 (1962).
Wang C. H., Stern I., Glimore C. M., Klungsoyr S., Reed D. J., Bialy J. J., Christensen B. E., Cheldlin V. H.: Comparative study of glucose catabolism by respirometric method.J. Bacteriol. 76, 207 (1958).
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Mian, F.A., Fencl, Z., Prokop, A. et al. Effect of growth rate on the glucose metabolism of yeast grown in continuous culture. Radiorespirometric studies. Folia Microbiol 19, 191–198 (1974). https://doi.org/10.1007/BF02895017
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DOI: https://doi.org/10.1007/BF02895017