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Electronmicroscopic comparison of the acid-fast and non-acid-fast strain ofMycobacterium phlei

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Abstract

Ultrastructure of the acid-fast PA strain ofMycobacterium phlei and its non-acid-fast PN mutant was studied in an electron microscope. Results of electronmicroscopic studies were supplemented with observation under an optical microscope, cultivation and chemical analysis of the wall mucopeptide. The effect of lysozyme and glycine on ultrastructure of cells of these strains was also followed. Electron microscopy revealed main differences in thickness and structure of cell walls, in intracellular membraneous system and in shape of rods of the studied strains.

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Authors and Affiliations

  1. Department of Bacterial Genetics and Department of General Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    M. Koníčková-Radochová, J. Čáslavská & J. Koníček

  2. Institute of Antibiotics and Biotransformations, 252 63, Roztoky near Prague

    F. Smékal

Authors
  1. M. Koníčková-Radochová
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  2. J. Čáslavská
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  3. F. Smékal
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  4. J. Koníček
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Koníčková-Radochová, M., Čáslavská, J., Smékal, F. et al. Electronmicroscopic comparison of the acid-fast and non-acid-fast strain ofMycobacterium phlei . Folia Microbiol 19, 183–190 (1974). https://doi.org/10.1007/BF02895016

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  • DOI: https://doi.org/10.1007/BF02895016

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