Abstract
Analysis of the peptidoglycan component of the cell wall was used as a means for identification of the genusActinomyces. The peptidoglycan ofActinomyces meyeri was of the lysine type, whereas that ofCorynebacterium granulosum isolated from the same infectious material contained diaminopimelic acid. Analysis according to Park and Hancock was found to be sufficient for taxonomic purposes. In samples containing both diaminopimelic acid and lysine the presence of lysine indicated contamination of the wall with residues of proteins.
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References
Bowden G. H., Hardie J. M.: Anaerobic organisms from the human mouth. In D. A. Shapton and R. G. Board: Isolation of anaerobes, p. 177, Academic Press, London-New York 1971.
Braun V., Sieglin U.: The covalent murein-lipoprotein structure of theEscherichia coli cell wall.J. Biochem.13, 336 (1970).
Cummins C. S., Glendenning O. M., Harris H.: Composition of the cell wall ofLactobacillus bifidus.Nature180, 337 (1957).
Cummins C. S.: Chemical composition and antigenic structure of cell walls ofCorrynebacterium, Mycobacterium, Nocardia, Actinomyces andArthrobacter.J. Gen. Microbiol.28, 35 (1962).
Cummins C. S.: Cell wall composition in the classification of grampositive anaerobes.Intarnat. J. System. Bacteriol.20, 413 (1970).
Hais J. M., Macek K.: Paper chromatography. (in Czech.) Publ. House Czech. Acad. Sci. (1954).
Holdeman L. V., Moore W. E. C.: Anaerobe laboratory manual. Polytechnic. Inst. State Univ. Blackburg, Vir. (1972).
Mariat F. M., Sebald M.: Actinomycoses (Actinomycoses à germes anaérobies. Nocardioses. Actinomycoses rares ou fausses actinomycoses) in Encyclopédie Médico-chirurgicale, Maladies infectieuses 8123 A10 (2) p. 8, Paris 1970.
Moore W. E. C.: Relationship of the metabolic product to taxonomy of anaerobic bacteria.Inter. J. Syst. Bacteriol.20, 535 (1970).
Park J. T., Hancock R.: Fractionation procedure studies of the synthesis of cell wall mucopeptide and of other polymers in cells ofStaphylococcus aureus.J. Gen. Microbiol.22, 249 (1960).
Perkins H. R.: Extraction procedures and cell wall composition including some results withCorynebacteria.Inter. J. Syst. Bacteriol.20, 379 (1970).
Pine L.: Classification and phylogenetic relationship of microaerophilic actinomyces.Intarnat. J. System. Bacteriol.20, 445 (1970).
Prévot A. R.: Techniques pour le diagnostic des bacteries anaérobies. 2nd Ed. Edition de la Tourelle, St. Mandé, 1966.
Prévot A. R., Turpin A., Kaiser P.: Les bactéries anaérobies, p. 1747, 1824, Dunod, Paris (1967).
Primosigh J., Pelzer H., Maas D., Weidel W.: Chemical characterization of mucopeptide released from theEscherichia coli B. cell wall by enzymic action.Biochem. Biophys. Acta46, 68 (1961).
Slack J. M., Gerencser M. A.: Range of cellular and colonial morphologies ofActinomyces israelli.Inter. J. System. Bacteriol.20, 259 (1970).
Work E.: The distribution of diaminoacids in cell walls and its significance in bacterial taxonomy.Inter. J. System. Bacteriol.20, 425 (1970).
Zierdt Ch. H.: Synonymy of species of Corynebacterium.Inter. J. System. Bacteriol.20, 23 (1970).
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Dedicated to the 60th birthday of Dr. L. Bíla, Director of the Thomayer Hospital
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Závadová, M., Chaloupka, J., Křečková, P. et al. Analysis of peptidoglycan as a means in diagnostics of the genusActinomyces . Folia Microbiol 19, 177–182 (1974). https://doi.org/10.1007/BF02895015
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DOI: https://doi.org/10.1007/BF02895015