Abstract
Two photooxidation products of indol-3-ylacetic acid (IAA), 3-hydroxymethyl oxindole (HMO) and 3-methylene oxindole (Meox) were almost as effective as IAA in stimulation of growth of oat (Avena saliva cv. Kent) coleoptile sections. The IAA failed to give stimulation of growth after pretreatment of the coleoptiles with chemicals inhibiting oxidation of IAA, but the growth promoting effects of HMO or Meox remained unaffected by such pretreatment. After pretreatment with the chemicals which can form an adduct with Meox, the growth promoting activities of IAA, HMO or Meox were lost showing the involvement of the oxindole pathway of IAA metabolism in IAA action. Time-course experiments also showed that the stimulatory effects of HMO and Meox were the same as IAA.
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Andersen, A. S., Møller, I., Hansen, J.: 3-Methyleneoxindole and plant growth regulation. -Physiol. Plant.27: 105–108, 1972.
Basu, P. S., Tuli, V.: Auxin activity of 3-methyleneoxindole in wheat. - Plant Physiol.50: 499–502, 1972a.
Basu, P. S., Tuli, V.: Enzymatic dehydration of 3-hydroxymethyloxindole. - Plant Physiol.50: 503–506, 1972b.
Chattopadhyay, K. K., Bhattacharyya, R. N., Basu, P. S.: Relation of gibberellic acid induced growth of wheat coleoptiles with indole acetic acid. - Biochem. Physiol. Pflanzen179: 123–128, 1984.
Evans, M. L.: Comparative effects of indole-3-acetic acid and 3-methylene oxindole on intact lentil root elongation. - Plant Sci. Lett.7: 447–453, 1976.
Frenkel, C.: Oxidative turnover of auxins in relation to the onset of ripening in Bartlett Pear. - Plant Physiol.55: 480–484, 1975.
Frenkel, C., Haddon, V. R., Smallheer, J. M.: Promotion of softening and ethylene synthesis in Bartlett Pears by 3-methyleneoxindole. - Plant Physiol.56: 647–649, 1975.
Fukuyama, T. T., Moyed, H. S.: Inhibition of cell growth by photooxidation products of indole-3-acetic acid. -J. biol. Chem.239: 2392–2397, 1964.
Ghosh, S., Basu, P. S.: Hormonal regulation of sex expression inMomordica charantia. - Physiol. Plant.57: 301–305, 1983.
Gordon, S. A., Weber, R. P.: Colorimetric estimation of indole acetic acid. - Plant Physiol.26: 192–195, 1951.
Grambow, H. J., Langenbeck-Schwick, B.: The relationship between oxidase activity, peroxidase activity, hydrogen peroxide and phenolic compounds in the degradation of IAAin vitro. - Planta157: 131–137, 1983.
Hinman, R. L., Bauman, C. P.: Reactions of 3-bromooxindoles. The synthesis of 3-methyleneoxindole. - J. org. Chem.29: 2431–2437, 1964.
Hinman, R. L., Lang, J.: Peroxidase catalyzed oxidation of indole-3-acetic acid. - Biochemistry4: 144–158, 1965.
Kar, M., Mishra, D.: Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. -Plant Physiol.57: 315–319, 1976.
Lau, O., John, W., Yang, S. F.: Inactivity of oxidation products of indole-3-acetic acid on ethylene production in mung bean hypocotyls. - Plant Physiol.61: 68–71, 1978.
Leopold, A. C., Kriedemann, P. E.: Plant Growth and Development. 2nd. Ed. - McGraw-Hill., Inc., New York 1975.
Lowry, O. H., Rosebrough, H. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. - J. biol. Chem.193: 265–275, 1951.
Meudt, W. J.: Electrophoretic isolation and growth activity of indole-3-acetic acid oxidation products. – In:Carr, D. J. (ed.): Plant Growth Substances 1970. Pp. 110–116. Springer-Verlag, Berlin 1972.
Pilet, P. E.: Le catabolism auxinique. - Bull. Soc. Fr. Physiol. vég.6: 119–137, 1960.
Ray, P. M., Thimann, K. V.: Steps in the oxidation of indole acetic acid. - Science122: 187–188, 1955.
Roberts, L. W.: Does 3-methyleneoxindole possess auxin activity? - J. exp. Bot.25: 761–763, 1974.
Sabater, F., Acosta, M., Sánchez-Bravo, J., Cuello, J., Delrio, J. A.: Indole-3-methanol as an intermediate in the oxidation of indole-3-acetic acid by peroxidase. - Physiol. Plant.57: 75–78, 1983a.
Sabater, F., Sánchez-Bbavo, J., Acosta, M.: Effects of enzyme-substrate ratio and of cofactors on the oxidation products of IAA catalyzed by peroxidase. - Rev. Esp. Fisiol.39: 169–174, 1983b.
Sinha, B. K., Basu, P. S.: Indole-3-acetic acid and its metabolism in root nodules ofPongamia pinnata (L) Pierre. - Biochem. Physiol. Pflanzen176: 218–227, 1981.
Sinha, B. K., Ghosh, S., Basu, P. S.: Auxin activity of 3-hydroxymethyl oxindole in wheat. -Bioahem. Physiol. Pflanzen176: 228–235, 1981.
Still, C. C., Olivier, C. C., Moyed, H. S.: Inhibitory oxidation products of indole-3-acetic acid: Enzymic formation and detoxification by pea seedling’s. - Science149: 1249–1251, 1965.
Tuli, V., Moyed, H. S.: The role of 3-methyleneoxindole in auxin action. - J. biol. Chem.244: 4916–4920, 1969.
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Bhattacharyya, R.N., Chattopadhyay, K.K. & Basu, P.S. Auxin activity of 3-hydroxymethyl oxindole and 3-methylene oxindole in oat. Biol Plant 28, 219–226 (1986). https://doi.org/10.1007/BF02894600
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DOI: https://doi.org/10.1007/BF02894600