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Effects of inhibitors on carotenoids biosynthesisin vivo inCapsicum annuum fruits

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Biologia Plantarum

Abstract

Diphenylamine (DPA), 2-hydroxybiphenyl (OHBP) and 9-fluorenone (9-F), known inhibitors of carotenoids (car) synthesis, altered the pattern of incorporation of 2-14C-mevalonate into car in ripening fruits ofCapsicum annuum. OHBP was the most powerful inhibitor of the whole process of biosynthesis, followed by DPA and 9-F. The most sensitive step was the dehydrogenation of phytofluene. Cyclization of β-carotene and isomerizations yielding the structure of hydroxy-cyclopentane were also affected, although to a lesser extent.

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References

  • Bramley, P. M., Davies, B. H.: β-carotene biosynthesis by extract of the C115 mutant ofPhycomyces blakesleeanus. - Phytochemistry15: 1913–1916, 1976.

    Article  CAS  Google Scholar 

  • Bucoltz, M. L., Maudinas, B., Porter, J.: Effects ofin vivo inhibitors of carotene biosynthesis on the synthesis of carotenes by a soluble tomato plastid enzyme system. - Chem.-Biol. Interactions17: 359–362, 1977.

    Article  Google Scholar 

  • Candela, M. E.: Doctoral Thesis. University of Murcia, Murcia 1982.

    Google Scholar 

  • Candela, M. E., Lopez, M., Sabater, F.: Carotenoids fromCapsicum annuum fruits: Changes during ripening and storage. - Biol. Plant.26: 410–414, 1984.

    Article  CAS  Google Scholar 

  • Clarke, I. E., de la Concha, A.,Murillo, F. J., Sandmann, G., Skone, E. J., Bramley, P. M.: The effect of diphenylamine on carotenogenesis inPhycomyces blakesleeanus. - Phytochemistry22: 435–439, 1983.

    Article  CAS  Google Scholar 

  • Davies, B. H., Matthews, S. S., Kirk, J. T. O.: The nature and biosynthesis of the carotenoids of different colour varieties ofCapsicum annuum. - Phytochemistry9: 797–805, 1970.

    Article  CAS  Google Scholar 

  • Goodwin, T. W.: Thirty years of biosynthesis. - Pure appl. Chem.51: 593–596, 1979.

    Article  CAS  Google Scholar 

  • Goodwin, T. W., Jamikorn, M., Willmer, J. S.: Studies in carotenogenesis. 7. Further observations concerning the action of DPA in inhibiting the synthesis of β-carotene inPhycomyces blakesleeanus. - Biochem. J.53: 531–538, 1953.

    PubMed  CAS  Google Scholar 

  • Goodwin, T. W., Land, D. G., Osman, H. G.: Carotenoid synthesis in the photosynthetic bacteriumRhodopseudomonas spheroides. - Biochem. J.59: 491–496, 1955.

    PubMed  CAS  Google Scholar 

  • Goodwin, T. W., Osman, H. G.: Studies in carotenogenesis. 10 Spirilloxanthin synthesis by washed cells inRhodospirillum rubrum. - Biochem. J.56: 222–226, 1954.

    PubMed  CAS  Google Scholar 

  • Gribanouski-Sassu, O.: Effect of DPA on carotenoid synthesis inDictyococcus cinnabarinus. - Phytochemistry11: 3195–3198, 1972.

    Article  Google Scholar 

  • De la Guardia, M. D.,Aragon, C. M. G., Murillo, F. J., Cerda-Olmedo, E.: A carotenogenic enzyme aggregate inPhycomyces: Evidence from quantitative complementation. - Proc. nat. Acad. Sci. USA9: 2012–2015, 1971.

    Article  Google Scholar 

  • Herber, R., Maudinas, B., Villoutreix, J., Granger, P.: Formation de phytoene all-trans chezMucor hiemalis. - Biochim. biophys. Acta280: 194–202, 1972.

    PubMed  CAS  Google Scholar 

  • Maudinas, B., Herber, R., Villoutreix, J., Granger, P.: Influence du 2-hydroxibiphényle sur la carotenogenèse deRhodopseudomonas spheroides et deRhodospirillum rubrum. - Biochemie54: 1085–1088, 1972.

    Article  CAS  Google Scholar 

  • Medina, J. R., Micol, J. L.: Carotenogenesis inPhycomyces blakesleeanus in the presence of 9-fluorenone. -Microbios Lett.16: 69–73, 1981.

    CAS  Google Scholar 

  • Oelze, J., Kamen, M. D.: Separation of respiratory reactions inRhodospirillum rubrum: Inhibition studies with 2-hydroxybiphenyl. - Biochim. biophys. Acta387: 1–11, 1975.

    Article  PubMed  CAS  Google Scholar 

  • Rilling, H. C.: A study of inhibition of carotenoid synthesis. - Arch. Biochem. Biophys.110: 39–46, 1965.

    Article  PubMed  CAS  Google Scholar 

  • Suzue, G.: Enzymatic synthesis of bacterial phytoene from 2-C14-mevalonic acid. - Biochim. biophys. Acta45: 616–617, 1960.

    Article  CAS  Google Scholar 

  • Valadon, L. R. G., Mummery, R. S.: Effect of certain inhibitors of carotenogenesis inVerticillium agaricinum.- Microbios7: 173–180, 1973.

    PubMed  CAS  Google Scholar 

  • Valadon, L. R. G., Mummery, R. S.: The effect of 9-fluorenone on protein synthesis and carotenogenesis inVerticillium aqaricinum. - Microbios6: 129–135, 1977.

    CAS  Google Scholar 

  • Than, A., Bramley, P. M., Davies, D. H., Rees, A. F.: Stereochemistry of phytoene.- Phytochemistry11: 3187–3192, 1972.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences, University of Murcia, 30001, Murcia, Spain

    M. E. Candela, M. Lopez & F. Sabater

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  1. M. E. Candela
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  2. M. Lopez
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  3. F. Sabater
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Candela, M.E., Lopez, M. & Sabater, F. Effects of inhibitors on carotenoids biosynthesisin vivo inCapsicum annuum fruits. Biol Plant 28, 196–201 (1986). https://doi.org/10.1007/BF02894596

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  • DOI: https://doi.org/10.1007/BF02894596

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