Skip to main content
SpringerLink
Log in

Marker enzymes of rat chemical hepatocarcinogenesis in human liver tumors

  • Article
  • Published:
Pathology & Oncology Research

Abstract

1st Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Budapest; Hungary Reduced glucose-6-phosphatase, increased GGT activity and reduction of cytochrome P-450 content are considered to be markers of chemical hepatocarcinogenesis in rats. The significance of these changes were studied in certain human liver lesions; adenoma, focal nodular hyperplasia and hepatocellular carcinoma all developed in noncirrhotic livers. Enzymes showed normal values in 4 out of 5 adenomas, in 2/13 FNH and in 4/18 HCC samples. The decreased cP-450 content in HCC proved to be the most consistent alteration (12/18). Only 3 HCC samples possessed changes off all enzymes. These data suggest that at least those enzymes which are used as markers in rat chemical hepatocarcinogenesis have little or no biological significance in human liver tumors, primarily due to the intertumoral heterogeneity of enzyme activity. Such heterogeneity was observed in the peritumoral “normal” liver tissue, too.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

cP-450:

cytochrome P-450

FNH:

focal nodular hyperplasia

GGT:

gamma glutamyl transpeptidase

G-6-Pase:

glucose 6 phophatase

HCC:

hepatocellular carcinoma

References

  1. Farber E: The sequential analysis of liver cancer induction. Biochim Biophys Acta 605:149–166, 1980.

    PubMed  CAS  Google Scholar 

  2. Phot HC and Sirica AE: The stages of initiation and promotion in hepatocarcinogenesis. Biochim Biophys Acta605:191–215, 1980.

    Google Scholar 

  3. Roomi MW, Ho RK, Sarma DSR and Farber E: A common biochemical pattern in preneoplastic hepatocyte nodules generated in four different models in the rat. Cancer Res 45:564–571. 1985.

    PubMed  CAS  Google Scholar 

  4. Mayer D, Moore M and Bannasch P: Biochemical correlation of glycogen content and activity of some enzymes of carbohydrate metabolism in rat liver during early stages of carcinogenesis. J Cancer Res Clin Oncol 104:99–108, 1982.

    Article  PubMed  CAS  Google Scholar 

  5. Kalengayi MMR and Dcsniet VJ: Gamma glutamyl transferase as oncofetal marker of experimental hepatocarcinogenesis in the rat. Scand J Immunol (Suppl) 8:547–556, 1978.

    CAS  Google Scholar 

  6. Szasz. G: A kinetic photometric method for serum γ-glutamyl transpeptidase. Clin Chem 15:124–136, 1969.

    PubMed  CAS  Google Scholar 

  7. Harper AE: Glucose-6-phosphatase. In: Bergmeyer HU. ed Methods of enzymatic analysis. Academic Press, New York, pp 788–792.

  8. Omura T and Sato R: The carbon monoxide binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 177:751–766. 1964.

    Google Scholar 

  9. Harlree EF: Determination of protein: modification of the Lowry method that gives linear photometric response. Anal Biochem 48:422–427, 1972.

    Article  Google Scholar 

  10. Taketa K, Shimamura J, Ueda M, Shimada Y and Kosaka K: Profiles of carbohydrate-metabolizing enzymes in human hepatocellular carcinomas and preneoplastic livers. Cancer Res 48:467–474, 1988.

    PubMed  CAS  Google Scholar 

  11. Nelson EB, Raj PP, Belfi KJ and Masters BSS: Oxidative drug metabolism in human liver microsomes. J Pharmac exp Ther 178:580–588. 1971.

    CAS  Google Scholar 

  12. Pelkonen O, Kaltiala EH, Larmi TKI and Karki NT: Cytochrome P-450 linked monooxygenase system and druginduced spectral interaction in human liver microsomes. Chem-biol Interact 9:205–216, 1974.

    Article  PubMed  CAS  Google Scholar 

  13. Selvaraj P and Balasubramanian KA: Soluble forms of γ- glutamyltransferase in human adult liver, fetal liver, and primary hepatoma compared. Clin Chem 29:90–95. 1983.

    PubMed  CAS  Google Scholar 

  14. Miyazaki M, Wahid S, Miyano K, Yabe T, Sato J: Expression of gamma-glutamyltranspeptidase in cultures of spontaneously and chemically transformed rat liver cells. Int J Cancer 32:373–377, 1983.

    Article  PubMed  CAS  Google Scholar 

  15. Jeney A: Trends in pathobiochemical studies of tumor development and progression. Acta Morph Hung 34:311–323. 1986.

    CAS  Google Scholar 

  16. Rao MS, Kokkinakis DM, Subbarao V and Reddy JK: Peroxisome proliferator-induced hepatocarcinogenesis: levels of activating and detoxifying anzymes in hepatocellular carcinomas induced by ciprofibrate. Carcinogenesis 8: 19–23,1987.

    Article  PubMed  CAS  Google Scholar 

  17. Kovalszky I, Szeberényi Sz, Zalatnai A, Vincze I.Lapis Kand Jeney A: Modification of DENA-induced hepatocarcinogenesis by CC14 cirrhosis. Comparison of the marker enzyme patterns. Carcinogenesis 13:773–778, 1992.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Pathology and Experimental Cancer Research, Semmelweis University of Medicine, Üllöi út 26, H-1085, Budapest, Hungary

    Ilona Kovalszky, Zsuzsa Schaff, Károly Lapis & András Jeney

Authors
  1. Ilona Kovalszky
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zsuzsa Schaff
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Károly Lapis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. András Jeney
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ilona Kovalszky.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kovalszky, I., Schaff, Z., Lapis, K. et al. Marker enzymes of rat chemical hepatocarcinogenesis in human liver tumors. Pathol. Oncol. Res. 2, 56–58 (1996). https://doi.org/10.1007/BF02893950

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02893950

Key words

Search

Navigation