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Detection of bladder cancer from the urine using fluorescencein situ hybridization technique

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Pathology & Oncology Research

Abstract

The authors report on their first experiences with the UroVysion fluorescencein situ hybridization (FISH) kit developed for the detection of bladder cancer. This new non-invasive diagnostic application of the FISH technique in the field of urology was elaborated to replace cystoscopy. The special urine examination method detects genetic alterations of the urothelial cells found in the urine, using fluorescent directlabeled DNA probes binding to the peri-centromeric regions of chromosomes 3, 7 and 17 as well as on the 9p21 locus. We aimed to evaluate the utility of UroVysion test in the light of the histological diagnosis. Urine samples from 43 bladder cancer patients and 12 patients with no or benign alterations were studied using a new application of FISH technique: the UroVysion reagent kit. The obtained FISH results were compared with the histological findings of the transurethral surgical resection specimens. The study rated the specificity and sensitivity of the technique 100% and 87%, respectively. Therefore, the technique could well fit into the diagnostic process of bladder carcinomas. Statistical analyses showed significant correlation between tumor progression and the severity of the genetic alterations detected by this FISH technique. Furthermore, positive correlation was found between tumor grade and the proportion of tumor cells showing genetic abnormality. The noninvasiveness, the robustness of evaluation and the high specificity/sensitivity are all in favor of this technique. The disadvantages are the higher costs of the technical background and the required future clinical studies to determine whether this technique can replace cystoscopy.

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Abbreviations

BTA test:

Bard’s bladder tumor antigen test

CCD:

charge-coupled device

CDKN2A:

cyclin-dependent kinase inhibitor 2A

CEP:

chromosome enumeration probe

CT:

computed tomography

DAPI:

4′,6-diamidino-2-phenylindole

FDA:

United States Food and Drug Administration

FISH:

fluorescence in situ hybridization

K-W/DMC tests:

Kruskal-Wallis / Dunn’s multiple comparison tests

LSI:

locus-specific identifier

MRI:

magnetic resonance imaging

MW test:

Mann-Whitney test

NMP22:

nuclear matrix protein 22

PBS:

phosphate-buffered saline

PCR:

polymerase chain reaction

SSC:

sodium chloride and sodium citrate solution

TPA:

tissue polypeptide antigen

UroVysion test-positive cells:

cells showing cytogenetic abnormalities listed in positivity criteria of UroVysion Bladder Cancer Recurrence Kit

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Authors and Affiliations

  1. Department of Urology, Semmelweis University, Budapest, Hungary

    Péter Riesz, Attila Szendrői, Attila Majoros & Imre Romics

  2. 2nd Department of Pathology, Semmelweis University, Üllői út 93, H-1091, Budapest, Hungary

    Gabor Lotz, Csilla Páska, Zsuzsanna Németh, Péter Törzsök, Zsuzsa Schaff & András Kiss

  3. 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

    Tibor Szarvas & Ilona Kovalszky

Authors
  1. Péter Riesz
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  2. Gabor Lotz
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  3. Csilla Páska
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  4. Attila Szendrői
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  5. Attila Majoros
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  6. Zsuzsanna Németh
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  7. Péter Törzsök
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  8. Tibor Szarvas
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  9. Ilona Kovalszky
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  10. Zsuzsa Schaff
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  11. Imre Romics
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  12. András Kiss
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Corresponding author

Correspondence to Gabor Lotz.

Additional information

Both authors have equally contributed to this work.

This work was supported by the following grants: NKFP-1A/0023/2002, OMFB 00606/2003

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Riesz, P., Lotz, G., Páska, C. et al. Detection of bladder cancer from the urine using fluorescencein situ hybridization technique. Pathol. Oncol. Res. 13, 187–194 (2007). https://doi.org/10.1007/BF02893498

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  • DOI: https://doi.org/10.1007/BF02893498

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