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Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α): Observations in cultured cells

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Pathology & Oncology Research

Abstract

The exact mechanism of the beneficial therapeutic action of interferon-a (IFN-α) in B-cell-lineage malignancies has not been adequately explained. Here we report on the differential effect of IFN-α2b on non-malignant B cells of umbilical cord blood and leukemic B-cell lines JY, BL-41 and BCBL-1. Leukemic cell proliferation was characterized by colony assay, whereas apoptosis was investigated by flow cytometry of propidium iodide-stained cells. The degree of differentiation was evaluated by measuring the expression level of Fcγ receptor-II (FcγRII) labeled with anti-CD32-FITC monoclonal antibody using flow cytometry. IFN-α protected umbilical cord blood CD19-positive B lymphocytes from apoptotic cell death in vitro. IFN-α significantly decreased colony formation of all three cell lines, and in contrast to normal cells, induced apoptosis in JY and BL-41 and excessive necrosis in HHV-8 infected BCBL-1 cells. FcγRII was upregulated both in normal and in leukemic B cells as indicated by an increase both in the proportion of CD32-positive cells and the mean fluorescence intensity. From our results it seems that antiproliferative, apoptotic and differentiative effects of IFN-a are interrelated but distinct cellular events, which are differentially regulated in normal, leukemic and virus-infected cells of the B-cell lineage. (Pathology Oncology Research Vol 12, No 3, 159–163)

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Abbreviations

FcγRII:

type II receptor for the constant fragment of immunoglobulin

G, FITC:

fluorescein-isothiocyanate

GM-CSF:

granulocytemacrophage colony-stimulating factor

HHV-8:

Human Herpesvirus 8

IFN-α:

interferon-alpha

IFN-γ:

interferon-gamma

IRF:

interferon regulatory factor

MFI:

mean fluorescence intensity

MNC:

mononuclear cell

PE:

phycoerythrin

SCF:

stem cell factor(c-kit ligand)

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Authors and Affiliations

  1. Department of Pediatrics, Debrecen, Hungary

    István Szegedi & Csongor Kiss

  2. Department of Clinical Biochemistry and Molecular Pathology, Debrecen, Hungary

    Éva Karászi

  3. Department of Cell Biophysics Research Group of the Hungarian Academy of Sciences, Debrecen, Hungary

    György Vámosi & János Szöllösi

  4. Department of Biophysics and Cell Biology, Debrecen, Hungary

    János Szöllösi

  5. Department of Pharmacology, Medical and Health Science Center, University of Debrecen, Hungary

    Péter Kovács & Ilona Benkő

Authors
  1. István Szegedi
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  2. Csongor Kiss
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  3. Éva Karászi
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  4. György Vámosi
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  5. János Szöllösi
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  6. Péter Kovács
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  7. Ilona Benkő
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Corresponding author

Correspondence to Csongor Kiss.

Additional information

I. Szegedi and C. Kiss contributed equally to this publication.

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Szegedi, I., Kiss, C., Karászi, É. et al. Differential regulation of umbilical cord blood and leukemic B cells by interferon-alpha (IFN-α): Observations in cultured cells. Pathol. Oncol. Res. 12, 159–163 (2006). https://doi.org/10.1007/BF02893363

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  • DOI: https://doi.org/10.1007/BF02893363

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