Abstract
Return-polyacrylamide gel electrophoresis (R-PAGE) and tomato bioassay followed by R-PAGE were compared for the detection of potato spindle tuber viroid (PSTVd) from individual true potato seed (TPS). Both methods detected PSTVd from single TPS. TPS extract formulated as sap or nucleic acids in two different buffers did not affect the percentage of viroid detection on tomato plants. There was some evidence of viroid inhibitor in TPS extracts but not in nucleic acid extracts of TPS. Because R-PAGE is more rapid than the tomato bioassay followed by R-PAGE, the former was used to determine the extent of PSTVd in TPS of China’s Keshan Potato Research Institute breeding material. Over 1700 individual TPS were tested. Twenty-four of the 46 seedlots tested (inbred and outcrossed) contained PSTVd. The viroid was detected in 70% of lots from inbred lines compared to 38% of lots from outcrosses. TPS (20 lots) stored in paper bags at room temperature as far back as 1965 were also tested, and PSTVd was detected in TPS stored for 21 years.
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This work was supported in part by the Canadian International Development Agency through the New Brunswick Department of Agriculture.
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Singh, R.P., Boucher, A. & Wang, R.G. Detection, distribution and long-term persistence of potato spindle tuber viroid in true potato seed from heilongjiang, China. American Potato Journal 68, 65–74 (1991). https://doi.org/10.1007/BF02893342
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DOI: https://doi.org/10.1007/BF02893342