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Picrotoxin-induced convulsions and lysosomal function in the rat brain

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Abstract

The effect of picrotoxin-induced convulsions on lysosomal function in rat brain were evaluated by measuring the free as well as total acid phosphatase, cathepsin D, acid ribonuclease (RNAse II) and acid deoxyribonuclease (DNAse II) activities. Following picrotoxin treatment the free RNAse II activity increased whereas the total activities of practically all the other enzymes decreased. Paradoxically, the cathepsin D activity, free as well the total was completely abolished. In case of all the enzymes the ratio of Total activity/Free activity decreased indicating increased lysosomal membrane fragility which could lead to process of neurodegeneration in the epileptic animals.

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References

  1. Sloviter, R.S. (1994) The functional organization of the hippocampal dentate gyrus and its relevance to the pathogenesis of temporal lobe epilepsy. Ann. Neurol. 35, 640–654.

    Article  PubMed  CAS  Google Scholar 

  2. Zola-Morgan, S., Squire, L. R. and Amaral, D. G. (1986) Human amnesia and the medial temporal region: enduring memory impairment following a bilateral lesion limited to field CA1 of the hippocampus. J. Neurosci. 6, 2950–2967.

    PubMed  CAS  Google Scholar 

  3. Zola-Morgan, S., Squire, L. R., Rempel, N. L. and Amaral D. G. (1992) Enduring memory impairment in monkeys after ischemic damage to the hippocampus. J. Neurosci. 12, 2582–2595.

    PubMed  CAS  Google Scholar 

  4. Singh R. (1980) Lysosomal acid phosphatase hyperactivity in cobalt epilepsy. Ind. J. Exp. Biol. 18, 716–719.

    CAS  Google Scholar 

  5. Sangiorgi, S., Ferlini, A., Zanetti, A. and Mochi, M. (1991) Reduced activity of arylsulfatase A and predisposition to neurological disorders: analysis of 140 pediatric patients. Am. J. Med. Genet. 40, 365–369

    Article  PubMed  CAS  Google Scholar 

  6. Tinuper, P., Plazzi, G., Monari, L., Sangiorgi, S., Pellissier, J. F., Cerullo, A., Provini, F., Capellari, S., Baruzzi, A. and Lugaresi, E. (1994) Arylsulfatase A pseudodeficiency and Lafora bodies in a patient with progressive myoclonic epilepsy. Epilepsia 35, 332–335.

    Article  PubMed  CAS  Google Scholar 

  7. Hetman, M., Filipkomski, R. K., Domagala, W. and Kaczmarck, L. (1995) Elevated cathepsin D expresson in kainate-evoked rat brain neurodegeneration. Exptl. Neurol. 136, 53–63.

    Article  CAS  Google Scholar 

  8. Houseweart M. K., Pennacchio L. A., Vilaythong A., Peters C., Noebels J. L., Myers R. M. (2003) Cathepsin B but not cathepsins L or S contributes to the pathogenesis of Unverricht-Lundborg progressive myoclonus epilepsy (EPM1). J Neurobiol. 56(4), 315–327.

    Article  PubMed  CAS  Google Scholar 

  9. Rinne R., Saukko P., Jarvinen M., Lehesjoki A. E. (2002) Reduced cystatin B activity correlates with enhanced cathepsin activity in progressive myoclonus epilepsy. Ann Med. 34(5), 380–385.

    Article  PubMed  CAS  Google Scholar 

  10. Mazarati, A. M., Halaszi, E. and Telegdy, G. (1992) Anticonvulsant effects of galanin administrated into the central nervous system upon the picrotoxin-induced seizures syndrome in rats. Brain Res. 589, 164–166.

    Article  PubMed  CAS  Google Scholar 

  11. Kubova, M., Bohuslav, T. and Mares, P. (1992) Effects of clonazepam on picrotoxin induced convulsions. Physiol. Res. 41, 167–169.

    PubMed  CAS  Google Scholar 

  12. Khandkar, M. A., Parmar, D. V., Das, M. and Katyare, S. S. (1996) Is activation of lysosomal enzymes responsible for paracetamol-induced hepatotoxicity and nephrotoxicity? J. Pharm. Pharmacol. 48, 437–440.

    PubMed  CAS  Google Scholar 

  13. Fiske C. H. and Subba Row Y. (1925) Colorimetric detemination of phosphorous. J Biol Chem. 66, 375.

    CAS  Google Scholar 

  14. Nerurkar, M. A., Satav, J. G. and Katyare, S. S. (1988) Insulin dependent changes in lysosomal cathepsin D activity in rat liver, kidney, brain and heart. Diabetologia 31, 119–122.

    Article  PubMed  CAS  Google Scholar 

  15. Barret, A. J. and Heath, M. F. (1977) Lysosomal enzymes, In: Lysosomes. A Laboratory handbook. Ed. Dingle, J. T., North Holland, Amsterdam, pp. 19–145.

    Google Scholar 

  16. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  17. Koike, M., Nakanishi, H., Saftig, P., Ezaki, J., Isahara, K., Ohsawa, Y., Schulz-Schaeffer, W., Watanabe, T., Waguri, S., Kametaka, S., Shibata, M., Yamamoto, K., Kominami, E., Peters, C., von Figura, K. and Uchiyama, Y. (2000) Cathepsin D deficiency induces lysosomal storage with ceroid lipofuchsin in mouse CNS neurons. J. Neurosci. 20, 6898–6906.

    PubMed  CAS  Google Scholar 

  18. Bernstein, H. G., Keilhoff, G., Kirschke, H., Wiederanders, B., Rinne, A., Khudoerkov, R. and Dorn, A. (1986) Cathepsins B and D in rat brain glia during experimentally induced neuropathological defects. An immunocytochemical approach. Biomed. Biochim. Acta. 45, 1461–1464.

    PubMed  CAS  Google Scholar 

  19. Olszewska, D., Drewa, T., Makarewicz, R. and Drewa, J., Wozniak, A. and Maciak, R. (2001) Significance of cathepsin B and D in physiologic and pathologic processes. Pol. Merkuriusz Lek. 10, 65–70.

    CAS  Google Scholar 

  20. Rostislav, K., Petr, B., Frantisek, C. and Oldrich, K. (2001) The cathepsin-D expression in the cytoinvasive stages of coccidia in intestinal mucosa of chickens, and the relevance of this proteinase for intracellular penetration and spreading of coccidian in host tissues. Biomed. Pap. Med. Fac. Univ. Palacky. Olomouc Czech Repub. 145, 2001.

    Google Scholar 

  21. Saftig, P., Hartmann, D., Lullmann-Rauch, R., Wolff, J., Evers, M., Koster, A., Hetman, M., von Figura, K. and Peters, C. (1997) Mice deficient in lysosomal acid phosphatase develop lysosomal storage in the kidney and central nervous system. J Biol. Chem. 272, 18628–35.

    Article  PubMed  CAS  Google Scholar 

  22. Tyynela, J., Sohar, I., Sleat, D. E., Gin, R. M., Donnelly, R. J., Baumann, M., Haltia, M. and Lobel, P. (2000) A mutation in the ovine cathepsin D gene causes a congenital lysosomal storage disease with profound neurodegeneration. EMBO J. 19, 2786–92.

    Article  PubMed  CAS  Google Scholar 

  23. Pope, A. and Nixon, R. A. (1984) Proteases of human brain. Brain Res. Rev. 14, 245–278.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, M.S. University of Baroda, 390002, Vadodara, Gujarat, India

    Munjal M. Acharya, Surbhi H. Khamesra & Surendra S. Katyare

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  1. Munjal M. Acharya
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  2. Surbhi H. Khamesra
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  3. Surendra S. Katyare
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Correspondence to Munjal M. Acharya.

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Acharya, M.M., Khamesra, S.H. & Katyare, S.S. Picrotoxin-induced convulsions and lysosomal function in the rat brain. Indian J Clin Biochem 20, 56–60 (2005). https://doi.org/10.1007/BF02893043

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