Virchows Archiv B

, Volume 39, Issue 1, pp 87–99 | Cite as

Observations on induced carcinosis peritonei of the guinea pig

  • Fred van de Molengraft
  • Paul Jap
  • Peter Wirtz
  • Peter Vooijs
Article

Summary

A diethylnitrosamine-induced hepatocellular carcinoma cell suspension (line-10), injected intraperitoneally in Sewall Wright strain-2 guinea pigs, causes ascites with implantation of malignant cells on the peritoneal surface. At these sites, swelling of the mesothelial cells and simultaneous proliferation of underlying fibroblasts and capillaries are seen. When there are about four layers of malignant cells and the mesothelial lining is disrupted, papillary projections of fibroblasts with capillaries, covered by malignant cells develop. These begin to behave as a “tissue”. In these areas basement membrane destruction and lymphatic and blood vessel infiltration are demonstrable. These developments have been investigated by light microscopy, histochemistry, transmission- and scanning electron microscopy.

Key words

Ascites Hepatocarcinoma Neoplasm metastasis Neoplasms experimental 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Fred van de Molengraft
    • 1
  • Paul Jap
    • 2
  • Peter Wirtz
    • 2
  • Peter Vooijs
    • 1
  1. 1.Section of Cytopathology, Institute for Pathologic AnatomyUniversity of NijmegenNijmegen
  2. 2.Department of Cytology and HistologyUniversity of NijmegenNijmegenThe Netherlands

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