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The effect of a single injection of cytosine arabinoside on cell population kinetics in the mouse jejunal crypt

Virchows Archiv B volume 34, Article number: 299 (1980) Cite this article

Summary

The early effects of a single injection of cytosine arabinoside (ara-C) on cell population kinetics in the jejunal crypt of the mouse were studied using autoradiography with tritiated thymidine, and metaphase arrest with vincristine. Ara-C had three main effects on crypt cells: a block of cells near the transition from G1 toS, death of nearly all cells inS, and a temporary block of the survivors, which remained viable and were able to proceed through the cell cycle. Throughout the crypt there was a decrease in cell cycle time and an increase in growth fraction. Although changes in proliferative rate were highest in the lowest part of the crypt it was not possible to show that crypt repopulation originated only from basal crypt cells, and the data are consistent with repopulation from the faster cycling cells in the proliferative compartment.

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References

  • Al-Dewachi HS, Wright NA, Appleton DR, Watson AJ (1974) The cell cycle time in the rat jejunal mucosa. Cell Tissue Kinet 7:599–606

    Google Scholar 

  • Al-Dewachi AS, Wright NA, Appleton DR, Watson AJ (1975) Cell population kinetics in the mouse jejunal crypt. Virchows Arch A Cell Path 18:225–242

    CAS  Google Scholar 

  • Al-Dewachi AS, Wright NA, Appleton DR, Watson AJ (1977) The effect of a single injection of hydroxyurea on cell population kinetics in the small bowel mucosa of the rat. Cell Tissue Kinet 10:203–213

    PubMed  CAS  Google Scholar 

  • Al-Dewachi HS, Wright NA, Appleton DR, Watson AJ (1979) Variation in the cell cycle time in the mouse jejunal crypt. Virchows Arch B Cell Path 31:37–44

    CAS  Google Scholar 

  • Boarder T, Blackett N (1976) The proliferative status of intestinal stem cells: Sensitivity to S phase cytotoxic agents. Cell Tissue Kinet 9:589–96

    PubMed  CAS  Google Scholar 

  • Cairnie AB (1976) Homeostasis in the small intestine. In: Cairnie AB, Lala PK, Osmond DG (eds) Stem cells of renewing cell populations. Academic Press, New York and London, p 67–77

    Google Scholar 

  • Cairnie AB, Lamerton LF, Steel GG (1965) Cell proliferation studies in the intestinal epithelium of the rat. Exp Cell Res 39:528–538

    PubMed  Article  CAS  Google Scholar 

  • Cheng H, Leblond CP (1974) Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine V Unitarian theory of the origin of the four epithelial cell types. Am J Anat 141:537–562

    PubMed  Article  CAS  Google Scholar 

  • Leblond CP, Cheng H (1976) Identification of stem cells in the small intestine of the mouse. In: Cairnie AB, Lala PK, Osmond DG (eds) Stem cells of renewing cell populations. Academic Press, New York and London, p 7–31

    Google Scholar 

  • Gilbert CW (1972) The labelled mitoses curve and the estimation of the parameters of the cell cycle. Cell Tissue Kinet 5:53–61

    PubMed  CAS  Google Scholar 

  • Graham FL, Whitmore GF (1970) The effect of 1-B-D-arabinofuranosylcytosine on growth, viability and DNA synthesis of mouse L cells. Cancer Res 30:2127–2131

    Google Scholar 

  • Hagemann RF, Sigdestad CP, Lesher S (1971) Intestinal crypt survival and total and per crypt levels of proliferative cellularity following irradiation: Single X-ray exposures. Radiat Res 46:533–546

    PubMed  Article  CAS  Google Scholar 

  • Lamerton LF (1972) Cell proliferation and the differential response of normal and malignant tissues. Br J Radiol 45:161–176

    PubMed  CAS  Article  Google Scholar 

  • Potten CS (1976) Small intestinal crypt stem cells. In: Cairnie AB, Lala PK, Osmond DG (eds) Stem cells of renewing cell populations. Academic Press, New York and London, p 79–84

    Google Scholar 

  • Potten CS, Al-Barwari SE, Harne WJ, Searle J (1977) Circadian rhythms of presumptive stem cells in three different epithelia of the mouse. Cell Tissue Kinet 10:557–568

    PubMed  CAS  Google Scholar 

  • Potten CS, Hendry J (1975) Differential regeneration of intestinal proliferative cells and cryptogenic cells after irradiation. Int J Radiat Biol 27:413–424

    Article  CAS  Google Scholar 

  • Tannock IF (1967) A comparison of the relative efficiencies of the various metaphase arrest agents. Exp Cell Res 47:315–329

    Article  Google Scholar 

  • Withers HR (1975) Cell cycle redistribution as a factor in multifraction irradiation. Radiat Biol 114:199–205

    CAS  Google Scholar 

  • Withers HR, Elkind MM (1970) Microcolony survival assay for cells of mouse intestinal mucosa exposed to radiation. Int J Radiat Biol 17:261–267

    Article  CAS  Google Scholar 

  • Wright NA, Morley AR, Appleton DR (1972) Variation in the duration of mitosis in the crypts of Lieberkuhn of the rat; A cytokinetic study using vincristine. Cell Tissue Kinet 5:351–362

    PubMed  CAS  Google Scholar 

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Author information

Affiliations

  1. Department of Pathology, University of Mosul, Iraq

    Huthaifa S. Al-Dewachi

  2. Department of Pathology, University of Oxford, England

    Nicholas A. Wright

  3. Department of Medical Statistics, University of Newcastle upon Tyne, England

    David R. Appleton

  4. Department of Pathology, University of Newcastle upon Tyne, England

    Alexander J. Watson

Authors
  1. Huthaifa S. Al-Dewachi
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  2. Nicholas A. Wright
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  3. David R. Appleton
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  4. Alexander J. Watson
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Al-Dewachi, H.S., Wright, N.A., Appleton, D.R. et al. The effect of a single injection of cytosine arabinoside on cell population kinetics in the mouse jejunal crypt. Virchows Archiv B Cell Pathol 34, 299 (1980). https://doi.org/10.1007/BF02892427

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  • DOI: https://doi.org/10.1007/BF02892427

Key words

  • Cytosine arabinoside
  • Cell proliferation
  • Intestine