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Nový způsob barvení mikroorganismů v půdních otiskových preparátech

Новый способ окраски микроорганизмов на п ластинках обрастания

A new method of staining micro-organisms in contact slide technique

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Резюме

Предлагаемый нами сп особ окраски удобен д ля фотографирования пр епаратов на пластинк ах обрастания. Предметн ые стекла, покрытые пл енкой агара или питательной сред ы, после инкубации в почве и просушивани я обрабатывают в тече ние 5 мин. фосфовольфрамовой к ислотой и окрашивают (5 мин.) кри сталлфиолетовой. Микроорганизмы окра шиваются очень контр астно.

Summary

The authors suggest a suitable staining method for photographing contact slide preparations. The slide is covered with an agar.film or nutrient substrate, incubated in the soil, and after drying is placed for five minutes in phosphotungstic acid and stained for a further five minutes with crystal violet. With this method the micro-organisms are well contrasted.

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Literatura

  • Conn, H. J.:The microsopic study of bacteria and fungi in soil. N. Y. Agr. Exp. Sta. Tech. Bul. 64, 1918.

  • Cholodnyj, N. G.:Über eine neue Methode zur Untersuchung der Bodenmikroflora. Arch. f. Mikrobiol. 1: 620, 1930.

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  • Rybalkina, A. V., Kononenko, E. V.:Aktivnaja mikroflora počv. Počvoveděnije 1956: 63.

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Authors and Affiliations

  1. Biologická fakulta Karlovy university, Praha a Botanická zahrada, Slovenská akademie věd, Košice

    Vera Kozderková & Jiří Bartoš

Authors
  1. Vera Kozderková
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  2. Jiří Bartoš
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Kozderková, V., Bartoš, J. Nový způsob barvení mikroorganismů v půdních otiskových preparátech. Československá Mikrobiologie 3, 58–60 (1958). https://doi.org/10.1007/BF02891756

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  • DOI: https://doi.org/10.1007/BF02891756

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