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Transport properties of membrane vesicles fromAcholeplasma laidlawii

II. Kinetic characteristics and specificity of glucose transport system

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Abstract

The glucose transport system of membrane vesicles isolated fromAcholeplasma laidlawii is saturable, with aK m of 21.2 μm andV of 0.68 nmol min−1 (mg protein)−1. The process is pH-dependent and a break occurs in the Arrhenius plot at 15°C. Exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-O-Methylglucose and 6-deoxyglucose competitively inhibited glucose transport. Maltose inhibited transport of glucose noncompetitively. These sugars also elicited glucose efflux from preloaded membrane vesicles.

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Authors and Affiliations

  1. Department of Biochemistry, Medical-Biological Faculty, 2nd Medical Institute, 21, Moscow, USSR

    L. F. Panchenko, N. S. Fedotov & M. A. Tarshis

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  1. L. F. Panchenko
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  2. N. S. Fedotov
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  3. M. A. Tarshis
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Panchenko, L.F., Fedotov, N.S. & Tarshis, M.A. Transport properties of membrane vesicles fromAcholeplasma laidlawii . Folia Microbiol 20, 480–487 (1975). https://doi.org/10.1007/BF02891707

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  • DOI: https://doi.org/10.1007/BF02891707

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