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A reappraisal of the stathmokinetic technique

I. An experimental study of a murine plasmacytoma with special reference to metaphase degeneration

  • Published:
Virchows Archiv B Cell Pathology Including Molecular Pathology

Summary

An “in vivo” study of metaphase degeneration in the murine plasmacytoma (MPC 11) over 8.5 h after mitotic arrest with vincristine sulphate and labelling with3H-TdR is described. The main points to emerge were that: (1) Metaphase degeneration becomes apparent from the decline in the unlabelled metaphase collection function after 3 to 4 h; (2) the decline is such that by 8.5 h approximately 25% of the metaphases which were present in the tissue at 3 h are no longer identifiable entities; (3) No circadian variation in the mitotic index was found; (4)3H-TdR may have a retarding effect on the entry of labelled cells into mitosis.

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Authors and Affiliations

  1. Department of Pathology, University of Newcastle upon Tyne, Great Britain

    D. Challoner

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  1. D. Challoner
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Challoner, D. A reappraisal of the stathmokinetic technique. Virchows Archiv B Cell Pathol 42, 95–110 (1983). https://doi.org/10.1007/BF02890373

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  • DOI: https://doi.org/10.1007/BF02890373

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