Summary
Endothelial cell membranes of rabbit carotid arteries were examined by the freeze-fracture technique. In the normal endothelium the mean densities of membrane-bound vesicles were 75 vesicles/μm2 on the luminal cell membrane and 102 vesicles/μm2 on the abluminal membrane. Whilst the vesical openings on the luminal membrane were randomly distributed those on the abluminal membrane were typically ordered in a macular pattern with lines free of vesicles. Tight and gap junctions between endothelial cells were numerous. After stimulating the carotid arteries with weak electrical impulses, a technique used to induce enhanced endothelial permeability and the formation of atheromatous plaques after repeated stimulations (Betz et al. 1985), vesicle openings were reduced to 78 vesicles/μm2 on abluminal membranes. Membranes on the luminal side and intercellular tight and gap junctions remained unchanged.
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References
Adamson RH, Huxley VH, Curry FE (1988) Single capillary permeability to proteins having similar size but different charge. Am J Physiol 254:H304-H312
Betz E, Hämmerle H, Strohschneider T (1985) Inhibition of smooth muscle cell proliferation and endothelial permeability with Flunarizine in vitro and in experimental atheromas. Res Exp Med 185:325–340
Betz E, Schlote W (1979) Responses of vessel walls to chronically applied electrical stimuli. Basic Res Cardiol 74:10–20
Bundgaard M (1983) Vesicular transport in capillary endothelium: Does it occur? Fed Proc 42:2425–2450
Chien S, Laufer L, Handley DA (1982) Vesicle distribution in the arterial endothelium determined with ruthenium red as an extracellular marker. J Ultrastruct Res 79:198–206
Clough G, Michel CC (1981) The role of vesicles in the transport of ferritin through frog endothelium. J Physiol 315:127–142
Curry FE, Huxley VH, Adamson RH (1983) Permeability of single capillaries of intermediate sized coloured solutes. Am J Physiol 245:H495-H505
Frokjaer-Jensen J, Wagner RC, Andrews SB, Hagman P, Reese TS (1988) Three-dimensional organization of the plasmalemmal vesicular system in directly frozen capillaries of the rete mirabile in the swim bladder of the eel. Cell Tissue Res 254:17–24
Ghinea N, Simionescu N (1985) Anionized and cationized hemeundecapeptides as probes for cell surface charge and permeability studies: Differentiated labeling of endothelial plasmalemmal vesicles. J Cell Biol 100:606–612
Hüttner I, Boutet M, More RH (1973) Studies on protein passage through arterial endothelium. I. Structural correlates of permeability in rat arterial endothelium. Lab Invest 28:672–677
Hüttner I, Walker C, Gabbiani G (1985) Aortic endothelial cell during regeneration: Remodeling of cell junctions, stress fibers and stress fiber-membrane attachment domains. Lab Invest 53:287–302
Karnovsky MJ (1967) The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol 35:213–236
Okuda T, Yamamoto T (1983) The ultrastructural basis of the permeability of arterial endothelium to horseradish peroxidase. Freeze-fracture and tracer studies of rat thoracic aorta and basilar artery. Cell Tissue Res 231:117–128
Palade GE, Bruns RR (1968) Structural modulations of plasmalemmal vesicles. J Cell Biol 37:633–653
Ross R, Glomset J, Harker L (1978) The responde to injury and atherogenesis. The role of endothelium and smooth muscle. Atheroscler Rev 3:69–76
Severs NJ (1988) Caveolae: static inpocketings of the plasma membrane, dynamic vesicles or plain artifact? J Cell Sci 90:341–348
Shasby DM, Shasby SS, Sullivan JM, Peach MJ (1982) Role of endothelial cell cytoskeleton in control of endothelial permeability. Circ Res 51:657–661
Shirama T, Cohen AS (1972) The role of mucopolysaccharides in vesicle architecture and endothelial transport. J Cell Biol 52:198–206
Simionescu M, Simionescu N, Palade GE (1974) Morphometric data on the endothelium of blood capillaries. J Cell Biol 60:128–152
Simionescu N (1983) Cellular aspects of transcapillary exchange. Physiol Rev 63:1536–1579
Wolburg H, Mühleisen H (1987) Gefrierbruchuntersuchungen an den Endothelzellen von arteriosklerotisch früh-veränderten sowie expantierten Gefäßabschnitten. In Hoffmeister, HE, Betz E (eds) Die Permeabilität der Gefäßwand. Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, pp 67–83
Wolff J, Merker JH (1966) Ultrastruktur und Bildung von Poren im Endothel von porösen und geschlossenen Kapillaren. Z Zellforsch 73:174–191
Yamamoto K, Fujimoto S, Takashige Y (1976) The fine structure of endothelial cells in freeze-fracture preparations. J Ultrastruct Res 54:22–28
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Mühleisen, H., Wolburg, H. & Betz, E. Freeze-fracture analysis of endothelial cell membranes in rabbit carotid arteries subjected to short-term atherogenic stimuli. Virchows Archiv B Cell Pathol 56, 413–417 (1988). https://doi.org/10.1007/BF02890045
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DOI: https://doi.org/10.1007/BF02890045