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Are all lymphoid blasts in the cell cycle?

DNA synthesis in the lymphoid tissues of the dog

Virchows Archiv B volume 56, Article number: 407 (1988) Cite this article

Summary

Labelling index after one or repeated intravenous injections of 3H-thymidine was measured for various subpopulations of lymphatic cells in different canine lymphoid compartments and correlated with cell morphology. High doses of tritiated thymidine were injected and exposure times of up to 211 days were used. The labelling indices of lymphoid blasts were comparable in all tissues investigated. Labelling index varied from 100% in immunoblasts to 4% in small-sized lymphocytes. Approximately 80% of immunoblasts were labelled 1 h after 3H-thymidine application and 100% labelling was obtained after 12 h repetitive 3H-thymidine labelling. In contrast with mediumsized and large lymphocytes, immunoblasts seem to be rapidly proliferating cells in the dog with almost no Go cells.

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Author information

Affiliations

  1. Klinik für Innere Medizin, Medizinische Universität zu Lübeck, D-2400, Lübeck, Federal Republic of Germany

    G. Wiedemann & T. Wagner

  2. Zentrum Anatomie, Medizinische Hochschule Hannover, D-3000, Hannover, Federal Republic of Germany

    R. Pabst

  3. Abt. Klinische Morphologie, Universität Ulm, D-7900, Ulm, Federal Republic of Germany

    F. Trepel

Authors
  1. G. Wiedemann
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  2. R. Pabst
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  3. T. Wagner
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  4. F. Trepel
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Supported by the Deutsche Forschungsgemeinschaft DFG Grant SFB 112

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Wiedemann, G., Pabst, R., Wagner, T. et al. Are all lymphoid blasts in the cell cycle?. Virchows Archiv B Cell Pathol 56, 407 (1988). https://doi.org/10.1007/BF02890044

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  • DOI: https://doi.org/10.1007/BF02890044

Key words

  • Lymphoid cell proliferation
  • 3H-thy-midine autoradiography
  • Dog