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Immunohistochemical analysis of normal and mutated ras oncogene p21 expression in human pulmonary and pleural neoplasms

Virchows Archiv B volume 56, pages 377–383 (1988)Cite this article

Summary

In this study we examined 214 cases of primary human pulmonary neoplasms for the expression of a mutated form of the ras oncogene p21 product, recognized by the monoclonal antibody (MCA) DWP. Adjacent serial sections from these same cases had previously been used to demonstrate the frequency of ras p21 expression using the broadly reactive anti-ras p21 MCA RAP-5. Confirmation of the increased expression of p21 was accomplished using MCA Yl3-259. The use of adjacent tissue sections from these cases allows the direct comparison of the expression of the mutated and non-mutated forms of ras p21. If reactivity with DWP would prove to be significantly more restrictive than that of the “pan” ras MCAs, RAP-5 and Yl3-259, it would lend support to the possibility that DWP (and similar MCAs which detect other specific mutations) could be used to define subsets of these neoplasms based on their specific ras p21 phenotype. Since one would anticipate that the valine/cysteine substitution at position 12 of the ras p21 would occur at only low frequencies in human tumors, our results with DWP are consistent with this hypothesis. As previously reported, RAP-5 reacted with a high proportion of lung tumors (100/214 or 47%). In this report, we demonstrate the selective expression of the mutation recognized by the MCA DWP in only 5% of these same tumors (13/214), and that the expression of this mutated form is not restricted to any of the conventional histological subclasses of pulmonary neoplasms.

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Abbreviations

MCA:

monoclonal antibody

SCC:

squamous cell carcinoma

AC:

adenocarcinoma

LCC:

large cell carcinoma

BAC:

bronchioloalveolar carcinoma

SCNC:

small cell neuroendocrine carcinoma

ICNC:

intermediate cell neuroendocrine carcinoma

WDNC:

well-differentiated neuroendocrine carcinoma

NE:

neuroendocrine

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Author information

Affiliations

  1. Department of Medicine, Section of Hematology/Oncology, Northwestern University/Veterans Administration Lakeside Medical Center, 303 East Chicago Avenue, 60611, Chicago, IL, USA

    J. A. Radosevich, Y. Ma & S. T. Rosen

  2. Department of Pathology, Rush-Presbyterian-St. Luke’s Medical Center, 1753 West Congress Parkway, 60612, Chicago, IL, USA

    V. E. Gould, I. Lee & W. H. Warren

  3. Laboratory of Tumor Immunology and Biology, National Institute of Health/NCI, Bethesda, Building 10 Room 8807, 20892, Maryland, USA

    A. Thor & J. Schlom

  4. Biomedical Products Department, E.I. DuPont de Nemours, Oncogene Research Group, 331 Treble Cove Road, 01862, North Billerica, Mass, USA

    W. P. Carney

Authors
  1. J. A. Radosevich
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  2. V. E. Gould
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  3. Y. Ma
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  4. I. Lee
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  5. A. Thor
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  6. W. P. Carney
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  7. W. H. Warren
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  8. J. Schlom
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  9. S. T. Rosen
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Additional information

This work was partly supported by a Veterans Administration Merit Review Grant to J.A.R and S.T.R., and by the Otho S.A. Sprague Memorial Fund to V.E.G.

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Radosevich, J.A., Gould, V.E., Ma, Y. et al. Immunohistochemical analysis of normal and mutated ras oncogene p21 expression in human pulmonary and pleural neoplasms. Virchows Archiv B Cell Pathol 56, 377–383 (1988). https://doi.org/10.1007/BF02890040

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  • DOI: https://doi.org/10.1007/BF02890040

Key words

  • Lung cancer
  • Immunoperoxidase
  • ras Oncogene
  • RAP-5
  • DWP
  • Y13-259
  • Monoclonal antibodies