Summary
The effects of cytologic sampling by contact-smear on the viability and morphology of epithelial tissues maintained as expiants in organ culture were studied. Tracheal and pancreatic tissues from Syrian golden hamsters were maintained for 30 days in culture, and sampled cytologically at weekly intervals. Analysis of sequential Papanicolaoustained smears demonstrates that expiant tissues can be evaluated cytologically over time in vitro without apparent effects on either the tissue viability, histochemistry or morphology. Microscopic examination further reveals that cellular samples retain diagnostically significant cytomorphologic features of the in vivo tissues. These observations indicate that this technique is useful for understanding the long-term response to carcinogens of epithelial tissue maintained in expiant organ culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Becci PJ, McDowell EM, Trump BF (1978) The respiratory epithelium. II. Hamster trachea, bronchus, and bronchioles. JNCI 61:551–561
Chopra DP (1982) Squamous metaplasia in organ cultures of vitamin A deficient hamster trachea: Cytogenetic and ultrastructural alterations. JNCI 69:895–905
Frost JK (1969) The cell in health and disease. Baltimore, Williams and Wilkins Co.
Frost JK, Erozan YS, Gupta PK (1983) Cytopathology of early lung cancer. In: Atlas of early lung cancer, National Cancer Institute. New York and Tokyo, Igaku-Shoin Medical Publishers, Inc.
Gill GW, Plowden KM (1985) Laboratory cytopathology: Techniques for specimen preparation. In: Frost JK (ed) Manual for the 26th Post-Graduate Institute for Pathologists in Clinical Cytopathology. Johns Hopkins University School of Medicine and Johns Hopkins Hospital, Baltimore
Hajdu S, Beam M, Fogh J, Hajdu E, Ricci A (1974) Papanicolaou smear of culture human tumor cells. Acta Cytol 18:321–332
Kennedy AR, Desrosiers A, Terzaghi M, Little JB (1978) Morphometric and histological analysis of the lungs of Syrian Golden hamsters. J Anat 125:527–553
Klein-Szanto AJP, Marchok AC, Terzhagi M (1985) In vivo studies on enhancement and promotion of respiratory tract carcinogenesis: Studies with heterotrophic tracheal transplants. In: Moss MJ, Kaufman DG, Siegfreid JM, Steele VE, Nesnow S (eds) Carcinogenesis. A Comprehensive survey, vol. 8, cancer of the respiratory tract: pre-imposing factors. Raven Press., New York, pp 119–130
Koss LG (1979) Diagnostic Cytology and its Histopathologic Bases (3rd Edition). Lippincott, Philadelphia
Luna LG (1968) Manual of Histological Staining Methods of the Aimed Forces Institute of Pathology (3rd Edition). McGraw-Hill Book Co., New York
McDowell EM, Trump BF (1976) Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol 100:405–414
Mossman BT, Kessler JB, Ley BW, Craighead JE (1977) Interaction of crocidolite asbestos with hamster respiratory mucosa in organ culture. Lab Invest 36:131–139
Murphy GF, Brody AR, Craighead JE (1980) Exfoliation of respiratory epithelium in hamster tracheal organ culture infected with mycoplasma pneumonia. Virchows Arch [Pathol Anat] 389:93–102
Resau JH, Jones RT (1984) Cytological studies of carcinogen treated bovine pancreatic ductal organ expiants. Virchows Arch [Cell Pathol] 45:355–363
Resau JH, Hudson EA, Jones RT (1983) Organ expiant culture of adult Syrian Golden hamster pancreas. In Vitro 19:315–325
Schreiber H, Nettesheim P (1972) A new method for pulmonary cytology in rats and hamsters. Cancer Res 32:737–745
Schreiber H, Saccomanno G, Morten DH, Brennan L (1974) Sequential cytologic changes during development of respiratory tract tumors induced in hamsters by benzo(a)pyrene ferric oxide. Cancer Res 34:689–698
Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:211–233
Sweat F, Puchtler H, Woo P (1964) A light fast modification of Lillie’s allochrome stain. Arch Pathol 78:73–75
Yoakum GH, Lechner JF, Gabrielson EW, Korba BE, Malan-Shibley L, Willey JC, Valerio MG, Shamsuddin AM, Trump BF, Harris CC (1985) Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene. Science 227:1174–1178
Valerio MG, Fineman EL, Bowman RL, Harris CC, Stoner GD, Autrup H, Trump BF, McDowell EM, Jones RT (1981) Long-term survival of normal adult human tissues as xenografts in congenitally athymic nude mice. JNCI 66:849–858
Author information
Authors and Affiliations
Additional information
This work was supported by a grant from the Maryland Division of the American Cancer Society, Inc.
Rights and permissions
About this article
Cite this article
Resau, J.H., Albright, C.D. Contact smear cytologic preparations of organ culture explant tissue. Virchows Archiv B Cell Pathol 52, 15–24 (1986). https://doi.org/10.1007/BF02889946
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02889946