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Control of CFUc proliferation by selective endogenous inhibitors

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Virchows Archiv B

Summary

The susceptibility of mouse bone marrow colony forming cells (CFUc) to three different types of proliferation inhibitors in capillary semisolid agar gel was studied. GI-3, a target specific peptide containing granulocyte fraction, T4-l, an oligospecific thymic factor of proteid nature, and the alkylating cytostatics dianhydrogalactitol (DAD) inhibit myeloid colony formation as a function of concentration. The respective MED values amount to 8, 10, and 0.002 μg/ml. When compared with this same parameter3H-TdR incorporation into DNA of liquid bone marrow cultures showed a single fold charge for the endogenous inhibitors (GI-3, T4-l) for the cytostatic (DAD) a 3 to 4 fold lower difference. It was demonstrated, that in competitive antagonism of GI-3 and colony stimulating factor the inhibitor prevails over CSF.

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  1. Institute of Experimental Medicine, Hungarian Academy of Sciences, Szigony U. 43, 1083, Budapest, Hungary

    A. Balázs & K. Barabás

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  1. A. Balázs
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  2. K. Barabás
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Balázs, A., Barabás, K. Control of CFUc proliferation by selective endogenous inhibitors. Virchows Archiv B Cell Pathol 31, 125–134 (1979). https://doi.org/10.1007/BF02889930

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  • DOI: https://doi.org/10.1007/BF02889930

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