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On serum as a mitogen in intact tissue

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Virchows Archiv B

Summary

The mitogenic effect of serum in defined cells in organ-cultured normal intact membranous rat mesentery was studied. Serum caused a strictly concentration-dependent stimulation of proliferation in mesothelial-cell-like and fibroblast-like cells, whereas the sequence S-G2-mitosis was apparently unaffected by serum. The results support the concept that similar mitogenic mechanisms may operate in cell culture and in situ in an intact tissue.

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References

  • Andersson, R.G.G., Norrby, K.: Induction of proliferation in dense non-starved 3T3 cells by Ca++ ionophore A23187. Virchows Arch. B Cell Path.23, 185–194 (1977)

    CAS  Google Scholar 

  • Baisch, H., Göhde, W., Linden, W.A.: Mathematical analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Rad. Environ. Biophys.12, 31–39 (1975)

    Article  CAS  Google Scholar 

  • Baserga, R.: Multiplication and division in mammalian cells. New York: Marcel Dekker, Inc., 1976

    Google Scholar 

  • Berridge, M.J.: Control of cell division: a unifying hypothesis. J. Cycl. Nucl. Res.1, 305–320 (1975)

    CAS  Google Scholar 

  • Crissman, H.A., Mullaney, P.F., Steinkamp, J.A.: Methods and applications of flow systems for analysis and sorting of mammalian cells. In: Methods in cell biology, D.M. Prescott (Ed.), vol. 9, pp. 179–246. New York: Academic Press, 1975

    Google Scholar 

  • DiPasquale, A., Jr. Bell, P.B.: The upper cell surface: its inability to support active cell movements in culture. J. Cell Biol.62, 198–214 (1974)

    Article  PubMed  CAS  Google Scholar 

  • Dean, P.N., Jett, J.H.: Mathematical analysis of DNA distribution derived from flow microfluorometry. J. Cell Biol.60, 523–527 (1974)

    Article  PubMed  CAS  Google Scholar 

  • Dulbecco, R., Elkington, J.: Conditions limiting multiplication of fibroblastic and epithelial cells in dense cultures. Nature (Lond.)246, 197–199 (1973)

    Article  CAS  Google Scholar 

  • Dulbecco, R., Elkington, J.: Induction of growth in resting fibroblastic cell cultures by Ca++. Proc. Nat. Acad. Sci. (Wash.)72, 1584–1588 (1975)

    Article  CAS  Google Scholar 

  • Enerbäck, L., Franzén, L., Norrby, K.: A tissue model for the study of cell proliferation parameters in vivo. Histochem.47, 207–218 (1976)

    Article  Google Scholar 

  • Franzén, L., Norrby, K.: Different proliferative responsiveness of fibroblasts and mesothelial cells in the rat mesentery following administration of compound 48/80 at various hours of the day. Virchows Arch. B Cell Path.24, 91–99 (1977)

    Google Scholar 

  • Fried, J.: Method for the quantitative evaluation of data from flow microfluorometry. Comp. Biomed. Res.9, 263–276 (1976)

    Article  CAS  Google Scholar 

  • Fried, J.: Analysis of deoxyribonucleic acid histograms from flow cytofluorometry. Estimation of the distribution of cells within S-phase. J. Histochem. Cytochem.25, 942–951 (1977)

    PubMed  CAS  Google Scholar 

  • Hassell, J., Engelhardt, D.L.: Factors regulating the multiplication of animal cells in culture. Exp. Cell Res.107, 159–167 (1977)

    Article  PubMed  CAS  Google Scholar 

  • Hodges, G.M.: An overview of tissue culture procedures in tumour biopsy studies. In: Human tumours in short term culture, P.P. Dendy (Ed.). pp. 3–15. London: Academic Press 1976

    Google Scholar 

  • Holley, R.W.: Control of growth of mammalian cells in cell culture. Nature (Lond.)258, 487–490 (1975)

    Article  CAS  Google Scholar 

  • Macdonald, P.D.M.: The distribution of DNA in exponentially-growing cell populations. In: Perspectives in probability and statistics-papers in honour of M.S. Bartlett on the occasion of his 65th birthday, J. Gani (Ed.), pp. 387–401. Applied Probability Trust, Sheffield, distributed by London: Academic Press, 1975

    Google Scholar 

  • McKeehan, W.L., Ham, R.G., Calcium and magnesium ions and the regulation of multiplication in normal and transformed cells. Nature (Lond.)275, 756–758 (1978)

    Article  CAS  Google Scholar 

  • Norrby, K., Franzén, L.: A tissue model for the study of cell proliferation in vitro. In Vitro, in press (1979)

  • Pledger, W.J., Stiles, CD., Antoniades, H.N., Scher, CD.: Induction of DNA synthesis in BALB/ c3T3 cells by serum components: reevaluation of the commitment process. Proc. Natl. Acad. Sci. (Wash.)74, 4481–4485 (1977)

    Article  CAS  Google Scholar 

  • Schultze, H.E., Heremans, J.F.: Molecular biology of human proteins. Amsterdam: Elsevier Publishing Co., 1966.

    Google Scholar 

  • Stoker, M.G.P.: Role of diffusion boundary layer in contact inhibition of growth. Nature (Lond.)246, 200–203 (1973)

    Article  CAS  Google Scholar 

  • Thrash, C.R., Cunningham, D.D.: Growth limitation of 3T3 mouse fibroblasts by available growth surface and medium components. J. Cell Physiol.86, 301–310 (1975)

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology II and Department of Mathematics, University of Linköping, S-581 85, Linköping, Sweden

    Klas Norrby & Anders Grimvall

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  1. Klas Norrby
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  2. Anders Grimvall
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Norrby, K., Grimvall, A. On serum as a mitogen in intact tissue. Virchows Archiv B Cell Pathol 31, 67–73 (1979). https://doi.org/10.1007/BF02889924

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  • DOI: https://doi.org/10.1007/BF02889924

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