Summary
Ultrastructural study of testicular biopsy specimens from an XX male showed hyalinized seminiferous tubules and tubules containing only mature Sertoli cells. These cells possessed large lipid inclusions as well as microfilament bundles which were perpendicular to the basement membrane and parallel to one another. The basal lamina was thickened and composed of several parallel layers with myofibroblast layers between them. The interstitium showed nodular to diffuse Leydig cell hyperplasia. Four types of Leydig cells were found: 1) normal Leydig cells with crystals of Reinke; 2) cells with abundant microcrystalline inclusions as well as microfilaments and concentric cisternae of smooth endoplasmic reticulum; 3) vacuolated cells containing numerous large lipid droplets; 4) immature Leydig cells. The different ultrastructural abnormalities found in the Sertoli and Leydig cells might be considered as the histological expression of a tubular-interstitial dysgenesis which is reflected in the high levels of gonadotropins and low levels of testosterone.
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References
Belt, W.D., Cavazos, L.F.: Fine structure of interstitial cells of Leydig in the squirrel monkey during seasonal regression. Anat. Rec.169, 115–128 (1971)
Burgos, M.H., Vitale-Calpe, R., Aoki, A.: Fine structure of the testis and its functional significance. In: The testis, A.D. Johnson, W.R. Gomes and N.L. Vandemark(eds.), vol. I, 551–649. New York: Academic Press 1970
Chakraborty, J., Nelson, L., Jhunjhunwala, J., Young, M., Kropp, K.: Basal lamina of the human seminiferous tubules-Its role in material transport. I. In presence of tunica vaginalis hydrocele. Cell Tiss. Res.174, 261–271 (1976)
Chapelle, A. de la, Hortung, H., Niemi, P., Wenstrom, J.: XX sex chromosomes in a human male: first case. Acta Med. Scand.412 (Suppl.), 25–38 (1964)
Chapelle, A. de la: Analytic review: Nature and origin of males with XX sex chromosomes. Am. J. Hum. Genet.24, 71–105 (1972)
Christensen, A.K., Wisner, J.R., Ort, J.: Preliminary observations on the localization of androgen and FSH receptors in the rat seminiferous tubule, studied by autoradiography at the light microscope level. In: The testis in normal and infertile men, P. Troen and H.R. Nankin (eds.), 153–171. New York: Raven Press 1977
Chung, K.W., Blackurn, W.R., Bullock, L.P., Santen, R.J., Bardin, C.W.: Leydig cell structure-function relationships in the mouse with XX sex reversal. In: The testis in normal and infertile men, P. Troen and H.R. Nankin (eds.), 45–55. New York: Raven Press 1977
Gravis, C.J., Weaker, F.J.: Testicular involution following optic enucleation. An ultrastructural and cytochemical study. Cell Tiss. Res.184, 67–77 (1978)
Grouchy, J. de, Canivet, J., Canlorbe, P.: Deux observations d’hommes 46,XX. Ann. Genet.10, 113–119 (1969)
Hard, W.L., Eisen, J.D.: A phenotipic male schwine with female karyotype. J. Hered.56, 254–258 (1965)
Ishida, H., Isurugi, K., Aso, Y., Takayasu, H., Tamakoi, B.I.: Endocrine studies in Sertoli-cell-only syndrome. J. Urol.116, 56–58 (1976)
Kovacs, K., Singer, W., Casal, G.: Leydig cell ultrastructure in an XX male. J. Urol.112, 651–654 (1974)
Kretser, D.M. de, Burger, H.G., Hudson, B.: The relationship between germinal cells and serum FSH levels in male infertility. J. Clin. Endocr. Metab.38, 787–793 (1974)
Kretser, D.M. de, Kerr, J.B., Paulsen, C.A.: The peritubular tissue in the normal and pathological human testis. An ultrastructural study. Biol. Reprod.12, 317–324 (1975)
Lindsten, J. Bergstrans, C.G., Tillinger, K.G., Schwaracher, H.C., Tiepolo, L., Muldal, S., Hokfelt,: A clinical and cytochemical study of three patients with male phenotype and apparent XX sex chromosome constitution. Acta Endocr.59, 91–112 (1966)
Mancini, R.E.: Bases histiofisiologicas de la funcion testicular. In: Testiculo humano (Ed. Fundacion para el fomento de estudios endocrinologicos de Rosario), 11–45. Buenos Aires: Panamericana 1968.
Neaves, W.B.: The blood-testis barrier. In: The testis, A.D. Johnson and W.R. Gomes (eds.), vol. IV, 125–162. New York: Academic Press 1977
Neaves, W.B.: Changes in testicular Leydig cells and in plasma testosterone levels among seasonally breeding rock ithyrax. Biol Reprod.8, 451–466 (1973)
Nicolis, G.L., Hsu, L.Y., Sabetgadam, R., Kardon, N.B., Chernay, P.R., Mathur, D.P., Rose, H.G., Hirschhorn, K., Gabrilove, J.L.: Klinefelter’s syndrome in identical twins with the 46,XX chromosome constitution. Am. J. Med.52, 482–491 (1972)
Nistal, M., Paniagua, R., Lopez-Pajares, L.: Ultrastructure of Leydig cells in Klinefelter’s syndrome with 48,XXYY karyotype. Virchows Arch. B Cell Path.28, 39–46 (1978)
Ohno, S.: Control of meiotic processes. In: The testis in normal and infertile men, P. Troen and H.R. Nankin (eds.), 1–87 New York: Raven Press 1977
Pais, V.M., Vasudevan, P.: Infertility in an XX male. J. Urol.118, 690–691 (1977)
Powers, H.O., Neu, R.L., Smulyan, H., Gadner, L.J.: An adult phenotipic male with a 46,XX chromosome complement. J. Clin. Endocr.31, 576–579 (1970)
Romani, F., Terquem, A., Dadoune, J.P.: Le testicule chez l’homme 46,XX: a propos d’une observation ultrastructural. J. Gyn. Obst. Biol. Rep.6, 1049–1059 (1977)
Sebaoun, M., Fournier, M., Dreyfus, G., Netter, A.: Les hommes de caryotype 46,XX. A propos de deux nouvelles observations. Ann. Endocr.30, 741–758 (1969)
Siew, S., Troen, P., Nankin, H.R.: Ultrastructure of testicular biopsies from infertile men. In: The testis in normal and infertile men, P. Troen and H.R. Nankin (eds.), 137–152. New York: Raven Press 1977
Steinberger, A., Steinberger, E.: The Sertoli cells. In: The testis, A.D. Johnson and W.R. Gomes (eds.), vol. IV, pp. 371–399. New York: Academic Press 1977.
Yamada, E.: Some observations on the fine structure of the interstitial cell in the human testis. In: 5th International Conference Electron Microscopy, S.S. Breese Jr. (ed.). vol. 2. New York: Academic Press 1962
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Nistal, M., Paniagua, R. Ultrastructure of testicular biopsy from an XX male. Virchows Archiv B Cell Pathol 31, 45–55 (1979). https://doi.org/10.1007/BF02889922
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DOI: https://doi.org/10.1007/BF02889922