Summary
Epithelial cell loss was induced in the descending colon of the rat by temporary ischaemia to investigate whether this would lead to an increase in crypt cell proliferation. Shortly after the temporary ischaemia the number of cells per crypt was markedly reduced, and it was shown that the cell loss occurred mainly from the non-proliferating upper half of the crypt. The number of cells per crypt reached control values again after 24–48 h. There was a marked increase in proliferative activity, as reflected by the labelling index after3HTdR and by the mitotic index, with peak values at 16 and 24 h after ischaemia. After 48 h the proliferative indices were normal again. The increase in crypt cell proliferation was characterized by an increase in the labelling index as well as in the mitotic index per crypt cell position. No enlargement of the proliferative cell compartment in the crypt was observed. It is most likely then that the increase in crypt cell proliferation was brought about by a shortening of the cell cycle, since the growth fraction in the lower half of the crypt approaches 1.0.
The possible implications of the present data for the control of colonic cell proliferation and colonic carcinogenesis are discussed.
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References
Brugal, G., Pelmont, J.: Existence of two chalone-like substances in intestinal tract from the adult newt, inhibiting embryonic intestinal cell proliferation. Cell Tissue Kinet.8, 171–187 (1975)
Cairnie, A.B.: Homeostasis in the small intestine. In: Stem cells, Cairnie, A.B., Lala, P.K., Osmond, D.G. (eds.), pp. 67–77. New York: Academic Press 1976
Cairnie, A.B., Millen, B.H.: Fission of crypts in the small intestine of the irradiated mouse. Cell Tissue Kinet.8, 189–196 (1975)
Chang, W.W.L., Nadler, N.J.: Renewal of the epithelium in the descending colon of the mouse. IV. Cell population kinetics of vacuolated-columnar and mucous cells. Am. J. Anat.144, 39–56 (1975)
Deschner, E.E., Lipkin, M.: Proliferative patterns in colonic mucosa in familial polyposis. Cancer35, 413–418 (1975)
Eastwood, G.L.: Gastrointestinal epithelial renewal. Gastroenterol.72, 962–975 (1977)
Galjaard, H., Meer-Fieggen, W. van der, Giesen, J.: Feedback control by functional villus cells on cell proliferation and maturation in intestinal epithelium. Exp. Cell Res.73, 197–207 (1972)
Hagemann, R.F., Stragand, J.J.: Fasting and refeeding: cell kinetic response of jejunum, ileum and colon. Cell Tissue Kinet.10, 3–14 (1977)
Hanson, W.R., Rijke, R.P.C., Plaisier, H.M., Ewijk, W. van, Osborne, J.W.: The effect of intestinal resection on Thiry-Vella fistulae of jejunal and ileal origin in the rat: evidence for a systemic control mechanism of cell renewal. Cell Tissue Kinet.10, 543–555 (1977)
Kanalingam, J., Houck, J.C.: Colon carcinoma: its genesis and chalone control. In: Chalones, Houck, J.C. (ed.), pp. 459–481. Amsterdam: North-Holland Puplishing Company 1976
Lipkin, M.: Proliferation and differentiation of gastrointestinal cells. Physiol. Rev.53, 891–915 (1973)
Lipkin, M.: Phase 1 and phase 2 proliferative lesions of colonic epithelial cells in premalignant diseases leading to colonic cancer. Cancer34, 878–888 (1974)
Mak, K.M., Chang, W.W.L.: Pentagastrin stimulates epithelial cell proliferation in duodenal and colonic crypts in fasted rats. Gastroenterol.71, 1117–1120 (1976)
Maskens, A.P.: Histogenesis and growth pattern of 1,2-dimethylhydrazine-induced rat colon adenocarcinoma. Cancer Res.36, 1585–1592 (1976)
Menge, H., Robinson, J.W.L., Riecken, E.O.: Anpassungsmöglichkeiten der Dünndarmschleimhaut an verschiedene intraluminale Milieuveränderungen. Z. Gastroenterol.14, 420–433 (1976)
Messier, B., Leblond, C.P.: Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am. J. Anat.106, 247–285 (1960)
Potten, C.S.: Knetics and possible regulation of crypt cell populations under normal and stress conditions. Bull. Cancer62, 419–430 (1975)
Pozharisski, K.M.: The significance of nonspecific injury for colon carcinogenesis in rats. Cancer Res.35, 3824–3830 (1975)
Robinson, J.W.L., Haroud, M., Winistöfer, B., Mirkovitch, V.: Recovery of the function and structure of dog ileum and colon following two hours’ acute ischaemia. Europ. J. Clin. invest.4, 443–452 (1974)
Robinson, J.W.L., Menge, H., Mirkovitch, V.: The response of the dog colon mucosa to one hour’s ischaemia. Res. Exp. Med.165, 127–134 (1975)
Rijke, R.P.C., Dongen, J.M. van: Control mechanisms of crypt cell production in the small intestinal epithelium. Acta Histochem., accepted for publication (1979)
Rijke, R.P.C., Hanson, W.R., Plaisier, H.M., Osborne, J.W.: The effect of ischemie villus cell damage on crypt cell proliferation in the small intestine: evidence for a feedback control mechanism. Gastroenterol.71, 786–792 (1976)
Rijke, R.P.C., Plaisier, H.M., Hoogeveen, A.T., Lamerton, L.F., Galjaard, H.: The effect of continuous irradiation on cell proliferation and maturation in small intestinal epitelium. Cell Tissue Kinet.8, 441–453 (1975)
Rijke, R.P.C., Plaisier, H.M., Langendoen, N.J.: Epithelial cell kinetics in the descending colon of the rat. Virchows Arch. B Cell Path.30, 85–94 (1979)
Sato, F., Maramatsu, S., Tsuchihashi, S., Shiragai, A., Hiraoka, T., Inada, T., Kawashima, K., Matsuzawa, H., Nakamura, W., Trucco, E., Sacher, G.A.: Radiation effects on cell populations in the intestinal epithelium of mice and its theory. Cell Tissue Kinet.5, 227–235 (1972)
Stragand, J.J., Hagemann, R.F.: Dietary influence on colonic cell renewal. Am. J. Clin. Nutr.3, 918–923 (1977)
Sunter, J.P., Wright, N.A., Appleton, D.R.: Cell population kinetics in the epithelium of the colon of the male rat. Virchows Arch. B Cell Path.26, 275–287 (1978)
Tutton, P.J.M.: Neural and endocrine control systems acting on the population kinetics of the intestinal epithelium. Med. Biol.55, 201–208 (1977)
Tutton, P.J.M., Barkla, D.H.: Cell proliferation in the descending colon of dimethylhydrazine treated rats and in dimethylhydrazine adenocarcinomata. Virchows Arch. B Cell Path.21, 147–160 (1976)