Summary
Ten nephroblastomas were investigated by antibodies to inter-mediate filaments. In seven cases, which in light microscopy were characterized by the presence of blastema and tubules, immunofluorescence microscopy with IF-specific antibodies reveals expression of cytokeratin and vimentin in blastema cells, while tubules were only labelled by the cytokeratin antibodies. This result was independent of whether the conventional cytokeratin antibody or monoclonal antibodies specific for cytokeratin 18 were used. Stroma cells were vimentin-positive. In two cases nephroblastomas were undifferentiated and also lacked tubuli formation. In both these tumors blastema cells were vimentin-positive and cytokeratin-negative. Finally one case of clear cell sarcoma of the kidney could only be labelled by the vimentin antibody. Thus antibodies to intermediate filaments seem to be useful tools to distinguish nephroblastomas from neuroblastomas or rhabdomyosarcomas, especially in cases of metastasis.
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Altmannsberger M, Osborn M, Treuner J, Hölscher A, Weber K, Schauer A (1982) Diagnosis of human childhood rhabdomyosarcoma by antibodies to desmin, the structural protein of muscle specific intermediate filaments. Virchows Arch [Cell Pathol] 39:203–215
Beckwith JB, Palmer NF (1978) Histopathology and prognosis of Wilm’s tumors. Results from the first national Wilm’s tumor study. Cancer 41:1937–1948
Bennett GS, Fellini SA, Toyama Y, Holtzer H (1979) Redistribution of intermediate filament subunits during skeletal myogenesis and maturationin vitro. J Cell Biol 82:577–584
Bodian M, Rigby CC (1968) The pathology of nephroblastoma. In: Riches E Sir (ed) Tumors of the kidney and ureter, vol 5. Williams and Wilkins, Baltimore, pp 219–234
Caselitz J, Osborn M, Scifert G, Weber K (1981) Intermediate-sized filament proteins (prekeratin, vimentin, desmin) in the normal parotid gland tumors. Virchows Arch [Pathol Anat] 393:273–286
Debus E, Weber K, Osborn M (1982) Monoclonal cytokeratin antibodies that distinguish simple from stratified human epithelia: characterization on human tissues. EMBO J 1:1641–1647
Franke WW, Schmid E, Osborn M, Weber K (1978) Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci USA 75:5034–5038
Franke WW, Schmid E, Schiller DL, Winter S, Jarasch E, Moll R, Denk H, Jackson BW, Illmensee K (1982) Differentiation patterns of expression of proteins of intermediate-sized filaments in tissues and cultured cells. Cold Spring Harbor Symp Quant Biol 46:431–453
Gabbiani G, Kapanci Y, Barazzone P, Franke WW (1981) Immunohistochemical identification of intermediate-sized filaments in human neoplastic cells. A diagnostic aid for the surgical pathologist. Am J Pathol 104:206–216
Jackson BW, Grund C, Winter S, Franke WW, Illmensee K (1981) Formation of cytoskeletal elements during mouse embryogenesis. Differentiation 20:203–216
Krepier R, Denk H, Artlieb U, Moll R (1982) Immunocytochemistry of intermediate filaments present in pleomorphic adenomas of the human parotid gland: characterization of different cell types in the same tumor. Differentiation 21:191–199
Lane EB, Hogan BLM, Kurkinen M, Garrels JI (1983) Co-expression of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo. Nature 303:701–704
Lehtonen E, Lehto VP, Paasivuo R, Virtanen I (1983) Parietal and visceral endoderm differ in their expression of intermediate filaments. EMBO J 2:1023–1028
Marsden HB, Lawler W, Kumar PM (1978) Bone metastasizing renal tumor of childhood. Morphological and clinical features, and differences from Wilms’ tumor. Cancer 42:1922–1928
Miettinen M, Lehto V-P, Badley RA, Virtanen I (1982) Expression of intermediate filaments in soft tissue sarcomas. Int I Cancer 30:541–546
Osborn M, Weber K (1982) Immunofluorescence and immunocytochemical procedures with affinity purified antibodies: Tubulin containing structures. In: Wilson L (ed) Methods in cell biology 24A:97-132
Osborn M, Weber K (1983) Biology of disease. Tumor diagnosis by intermediate filament typing: A novel tool for surgical pathology. Lab Invest 48:372–394
Osborn M, Geisler N, Shaw G, Sharp G, Weber K (1982a) Intermediate filaments. Cold Spring Harbor Symp Quant Biol 46:413–429
Osborn M, Altmannsberger M, Shaw G, Schauer A, Weber K (1982b) Various sympathetic derived human tumors differ in neurofilament expression. Virchows Arch [Cell Pathol] 40:141–156
Osborn M, Debus E, Altmannsberger M, Weber K (1983) Uses of conventional and monoclonal antibodies to intermediate filament proteins in the diagnosis of human tumors. Proc. 4th Internat Expert Meeting of the Deutsche Stiftung für Krebsforschung [in press]
Ramaekers FCS, Haag D, Kant A, Moesker O, Jap PHK, Vooijs GP (1983) Coexpression of keratin and vimentin-type intermediate filaments in human metastatic carcinoma. Proc Natl Acad Sci USA 80:2618–2622
Schmidt D, Dickersin RG, Vawter GF, Mackay B, Harms D (1982) Wilms’ tumor: Review of ultrastructure and histogenesis. In: Joachim HL (ed) Pathobiology Annuals 1982, vol 12. Raven Press, New York, pp 281–300
Sharp G, Osborn M, Weber K (1982) Occurrence of two different intermediate filament proteins in the same filament in situ within a human glioma cell line. Exp Cell Res 141:385–395
Shaw G, Weber K (1981) The distribution of the neurofilament triplet proteins within individual neurones. Exp Cell Res 136:119–125
Willis RA (1977) Pathology of tumors. Butterworth, London, 4th ed, pp 944–951
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Altmannsberger, M., Osborn, M., Schäfer, H. et al. Distinction of nephroblastomas from other childhood tumors using antibodies to intermediate filaments. Virchows Archiv B Cell Pathol 45, 113–124 (1984). https://doi.org/10.1007/BF02889858
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DOI: https://doi.org/10.1007/BF02889858