Abstract
The DNA damaging effects of the carbamate pesticide carbofuran and its four metabolites (carbofuranphenol, 3-ketocarbofuran, 3-hydrocarbofuran and nitrosocarbofuran) on mice were evaluated by single cell gel electrophoresis (SCGE) assay and micronucleus test. KM mice were exposed to test compounds with different doses of 0.1, 0.2 and 0.4mg/kg through intraperitoneal injection two times with an internal of 24 h, and then killed by cervical dislocation 6 h after the second injection. In SCGE assay, isolated mice peripheral blood lymphocytes were employed to determine DNA damaging degree after a 1 h treatment by test compounds and a following electrophoresis. Carbofuran and carbofuranphenol showed negative results in both test and had no obvious toxicity. 3-hydrocarbofuran and nitrosocarbofuran were positive. 3-ketocarbofuran could not induce micronucleus formation but caused significant DNA migration in SCGE test. These tests revealed that 3-ketocarbofuran, 3-hydrocarbofuran and nitrosocarbofuran are potential mutagesis and further research is needed.
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Rawlings, N. C., Cook, S. J., Waldbillig, D., Effects of the pesticides carbofuran, chlorpyrifos, dimetholate, lindane, triallate, trifluralin, 2,4-D, and pentachlorophenol on the metabolic endocrine and reproductive endocrine system in ewes, J. Toxico. Environ. Health, 1998, 54: 21–36.
Cole, D. C., Carpio, F., Julian, J. et al., Assessment of peripheral nerve function in an Ecuadorian rural population exposed to pesticides, J. Toxicol. Environ. Health., 1998; 55: 77–91.
Peter, J. V., Cheriam, A. M., Organic insecticides., Anaesth. Intensive Care, 2000, 28: 11–21.
Kamel, F., Boyes, W. K., Gladen, B. C. et al., Retinal degeneration in licenced pesticide applications, Am. Ind. Med., 2000, 37: 618–628.
Winston, H. H., Public health goal for chemicals in drinking water: carbofuran, California Environmental Protection Agency, 2000.
Ferguson, P. W., Dey, M. S., Jewell, S. A., et al., Carbofuran metabolism and toxicity in the rat, Fundamental and Applied Toxicology, 1984, 4: 14–21.
Ostling, R. N., Johanson, K. J., Microelectrophotetic study of radiation induced DNA damages in individual mammalian cells, Biochen. Biophys. Res. Commun., 1984; 123: 291–298.
Singh, N.P., Mecoy, M.T., Tice, B.R. et al., A simple technique for quantitation of low levels of DNA damage in individual cells, Experimental Cell Research, 1988, 175: 184–191.
Vigreux, J. M., Doul, E., Deslandes, C. et al., DNA damaging effects of pesticides measured by the single cell gel electrophoresis assay (comet assay) and the chromosomal aberration test, in CHOK1 cells, Mutation Research, 1998, 419: 79–90.
Chauvel, D. J., Auroy, P., Tricot, S. et al., Evaluation of the capacity of the SCGE assay to assess the genotoxicity of biomaterials, Biomaterials, 2001, 22: 1795–1801.
Dana, D. K., Saleha, B. B., Paramjit, G. et al., Genotoxic effect of lead nitrate on mice using SCGE (comet assay), Toxicology, 2000, 145: 195–201.
Robert, W. R., Gerd, W. E. et al., Importance of pH in assessing the potential for nitrosocarbamate formation in the stomach, Pesticide Biochemistry and Physiology, 1982, 8: 325–333.
Ji-Young Yoon, Seon-Hee Oh, Su-Mi Yoo, et al., N-Nitrosocarbofuran, but not carbofuran, induces apoptosis and cell cycle arrest in CHL cells, Toxicology, 2001, 169: 153–161.
Nelson, J., MacKinnon, E. A., Mower, H. F. et al., Mutagenicity of N-nitroso derivatives of carbofuran and its toxic metabolites, J. Toxicol. Environ. Health, 1981, 7: 519–531.
Wang, T. C., Chiou, C. M., Chang, Y.L., Genetic toxicity of N-methylcarbamate insecticides and their N-nitroso derivatives, Mutagenesis, 1998, 13: 405–408.
Lijinsky, W., Schmaehl, D., Carcinogenicity of N-nitroso derivatives of N-methylcarbamate insecticides in rats, Ecotoxico.l Environ. Saf, 1978, 2: 413–419.
Wang, T.C., Chiou, J. M., Chang, Y.L. et al., Genotoxicity of propuxur and its N-nitroso dirivative in mammalian cells, Carcinogenesis, 1998, 19: 623–629.
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Zhou, P., Liu, B. & Lu, Y. DNA damaging effects of carbofuran and its main metabolites on mice by micronucleus test and single cell gel electrophoresis. Sci. China Ser. C.-Life Sci. 48 (Suppl 1), 40–47 (2005). https://doi.org/10.1007/BF02889800
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DOI: https://doi.org/10.1007/BF02889800