Skip to main content
SpringerLink
Log in

Impacts of methamidophos, copper, and their combinations on bacterial community structure and function in black soil

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

The potential ecotoxicologial risks of methamidophos, copper, and their combinations on microbial community of black soil ecosystem in the Northeast China were assessed in species richness and structures by using 16S rDNA-PCR-DGGE analysis approach, and functional characteristics at community levels by using BIOLOGGN system analysis method as well as two conventional methods(DHA and SIR). All results of DGGE banding fingerprint patterns(amplified by bacterial specific 16S rDNAV3 high variable region universal primer) indicated that the species richness of bacterial community in tested soil was significantly decreased to different extents by using different concentrations of single methamidophos, copper, especially some of their combinations had worse effects than their corresponding single factors. In addition, the structures of soil bacterial community had been disturbed under all stresses applied in this study because of the enrichment of some species and the disappearance of other species from the bacterial community. The effects of the single factors with lower concentrations on the community structure were weaker than those with higher concentrations. Moreover, the bacterial community structures under the combined stresses of methamidophos and copper were significantly different from those of control and their corresponding single factors. The change of DHA and carbon source substrate utilizing fingerprint patterns based on BIOLOGGNsystem were two relatively sensitive directors corresponding to the stress presented in this study. Between methamodophos and copper, there happened the significant joint-toxic actions when they were used in combination on DHA and carbon source substrate utilizing fingerprint patterns of soil bacterial communities. The DHA of soil under the combined stresses was lower than that of the control and that under the single factors, and the BIOLOGGN substrate utilizing patterns of soil treated by combinations were distinctively differentiated from the control and their corresponding single factors. From all of above, the methamidophos, copper, especially their combinations had the clearly potential ecotoxicological risks to influence the natural soil microbial ecological system by changing the structure, richness, and the functional characteristics of microbial community.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kennedy, A. C., Smith, K. L., Soil microbial diversity and the sustainability of agrochultural soils, Plants and Soil, 1995, 170: 75–86.

    Article  CAS  Google Scholar 

  2. Finlay, B. J., Maberly, S. C., Cooper, J. I., Microbial diversity and ecological function, Oikos, 1997, 80: 209–213.

    Article  Google Scholar 

  3. Groffman, P. M., Bohlen, P. J., Soil and sediment biodiversitycross-system comparisons and large-scale effects, BioScience, 1999, 49: 130–148.

    Article  Google Scholar 

  4. Kandeler, E., Tscherko, D., Bardgett, R. D. et al., The response of microorganisma and roots to elevated CO2 and temperature in a terrestrial model ecosystem, Plant and Soil, 1998, 202: 251–262.

    Article  CAS  Google Scholar 

  5. Ibekwe, A. M., Papiernik, S. K., Gan, J.Y. et al., Impact of fumigants on soil microbial communities, Appl. Environ. Microbiol., 2001, 67(7): 3245–3257.

    Article  PubMed  CAS  Google Scholar 

  6. Chang, Y. J., Hussain, A.K., Stephen, J. R. et al., Impact of herbicides on the abundance and structure of indigenous beta-subgroup ammonia-oxidizer communities in soil microcosms, Environ. Toxicol. Chem., 2001, 20(11): 2462–2468.

    Article  PubMed  CAS  Google Scholar 

  7. Kaare, J., Carsten, S., Jacobsen, V. T. et al., Review article: Pesticide effects on bacterial diversity in agriccultural soils, Biology and Fertility of Soil, 2001, 33(6): 443–453.

    Article  Google Scholar 

  8. Said, E. F., Laurent, V., Willy, V. et al., Effect of phenylurea herbicides on soil microbial communities estimated by analysis of 16S rRNA gene fingerprints and community-level physiological profiles, Appli. Eviron. Microbiol., 1999, 65(3): 982–988.

    Google Scholar 

  9. Morgan, D. P., U.S. Environmental Protection Agency, Recognition and Management of Peaticide Poisonings, 4th ed., Washonton, DC: Health Effects Division, Office of Pesticide Programs, U.S. EPA, 1989.

    Google Scholar 

  10. Juarez, L. M., Sanchez, J., Toxicity of the organophosphorus insecticide methamidophos to larvae of the freshwater prawnMaceobrachium rosenbergii (De Man) and the slue shrimpPenaeus stylirostris Stimpson, Bull. Environ. Contam. Toxicol., 1989, 43 302–309.

    Article  PubMed  CAS  Google Scholar 

  11. U.S. Environmental Protection Agency, Pesticide Environmental Fate On Line Summary: Methamidophos, Washington, DC: U.S. EPA Environmental Fate and Effects Division, 1989.

    Google Scholar 

  12. Garland, J. L., Mills, A. L., Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level sole-carbon-source utilization, Appli, Environ. Microbiol., 1991, 57(8): 2351–2359.

    Google Scholar 

  13. De Fede, K. L., Sexstone, A. J., Differential response of size-fractioated soil bacteria in BIOLOG® microtitre plates, Soil Biology and Biochemistry, 2001, 33(11): 1547–1554.

    Article  Google Scholar 

  14. Choi, K. H., Dobbs, F. C., Comparison of two kinds of biolog microplates(GN and ECO) in their ability to distinguish among aquatic microbial communities, Journal of Microbilogical Methods, 1999, 36(3): 203–213.

    Article  CAS  Google Scholar 

  15. Kaiser, S. K., Guckert, J. B., Gledhill, D. W., Comparison of activated sludge microbial communities using biolog™ microplates, Water Science and Technology, 1998, 37(4–5): 57–63.

    Article  CAS  Google Scholar 

  16. Engelen, B., Meinken, K., Wintzingerode, F. et al., Monotoring impact of a pesticide treatment on bacteria soil communities by metabolic and genetic fingerprinting in addition to conventional testing procedures, Appli. Environ. Microbiol., 1998, 64(8): 2814–2821.

    CAS  Google Scholar 

  17. Muyzer, G., De Waal, E. C., Uitterlinden, A. G., Profiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding 16S rRNA, Appl. Eviron. Microbiol., 1993, 59: 695–700.

    CAS  Google Scholar 

  18. Lu Rukun, Analysis Methods of Soil Agrochemistry, Beijing: Agricultural Science and Technology Press, 1999

    Google Scholar 

  19. Yang Yonghua, Yao Jian, Hua Xiaomei, Effects of agrochemicals on functional diversity of microbial community in soil, Journal of Microbiology, 2000, 20(2): 23–26.

    Google Scholar 

  20. Kuske, C. R., Banton K. L., Asorada, D. L. et al., Small-scale DNA sample preparation method for field PCR detection of microbial cell and spores in soil, Appli. Environ. Microbiol., 1998, 64(7): 2463–2472.

    CAS  Google Scholar 

  21. Koshikawa, O. H., Nakahara, T., Uchiyama, H., Succession of microbial communities during a biostimulation process as evaluated by DGGE and clone library analyses, Journal of Applied Microbiology, 2001, 91: 625–635.

    Article  PubMed  Google Scholar 

  22. Bassam, B. J., Caetano-Anollés, G., Fast and sensitive silver staining of DNA in polyacrylamide gels, Annual Biocchemistry, 1991, 196: 80–83.

    Article  CAS  Google Scholar 

  23. Biodiversity Committee of the Chinese Academy of Science, Biodiversity Studies Series No.1, Principles and Methodologies of Biodiversity Studies, Beijing: Chinese Science and Technology Press, 1994, 141–165.

    Google Scholar 

  24. Zhang, H.W., Zhang, Q.R., Zhou, Q.X. et al., Introduction and progress of molecular microbial ecology, Chinese Journal of Applied Ecology, 2003, 14(2): 286–292.

    PubMed  CAS  Google Scholar 

  25. Wünsche, L., Brüggermann, L., Wolfgang, B., Determination of structure in utilization patterns of soil microbial communities: An approach to assess population changes after hydrocarbon pollution, FEMS Microbiol. Ecol., 1995, 17: 295–306.

    Google Scholar 

  26. Zhang, H., Zhang, Q., Zhou, Q. et al., Binary-joint effects of acetochlor, methamidophos, and copper on soil microbial population, Bull. Envrion. Contamin. Toxicol., 2003, 71: 746–754.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Science, 110016, Shenyang, China

    Zhang Huiwen, Qixing Zhou, Qianru Zhang & Chenggang Zhang

Authors
  1. Zhang Huiwen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qixing Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qianru Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chenggang Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhang Huiwen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H., Zhou, Q., Zhang, Q. et al. Impacts of methamidophos, copper, and their combinations on bacterial community structure and function in black soil. Sci. China Ser. C.-Life Sci. 48 (Suppl 1), 14–25 (2005). https://doi.org/10.1007/BF02889797

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02889797

Keywords

Search

Navigation