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Die Feinstruktur der Gallenblase und der Gallengänge

IV. Die Cholesterose der menschlichen Gallenblasenschleimhaut

On the fine structure of gall bladder and biliary ducts

IV. The cholesterosis of the human gall bladder mucosa

  • Published:
Virchows Archiv B

Summary

The fine structure of the mucosa of surgically removed gall-bladders was studied in three cases of cholesterosis with both thin-section and freeze-etching methods.

The principal cells of the epithelium exhibit a well developed smooth surfaced endoplasmic reticulum which is probably related to lipid transport. An abundance of macrophages is found between the epithelial cells and in the lamina propria. At places, they penetrate the basement membrane. Some of the intraepithelial macrophages show flattened smooth surfaced cisternae connected with profiles of the rough surfaced endoplasmic reticulum. In other macrophages, located adjacent to the epithelium in the lamina propria, the smooth surfaced cisternae are more numerous and larger. With increasing distance from the epithelium these profiles in the macrophages become larger and finally reach the size and appearance of foam cell vacuoles. Freeze-etching replicas demonstrate the “vacuoles” of the foam cells to contain lipids.

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Literatur

  • Bader, G.: Die submikroskopische Struktur des Gallenblasenepithels. III. Mitteilung: Das Epithel der Steingallenblase des Menschen. Frankfurt. Z. Path.74, 501–511 (1965)

    PubMed  CAS  Google Scholar 

  • Cardeil, R. R., Jr., Badenhausen, S., Porter, K. R.: Intestinal triglyceride absorption in the rat. An electron microscopical study. J. Cell Biol.34, 123–155 (1967)

    Article  Google Scholar 

  • Chapman, G. B., Chiarodo, A. J., Coffey, R. J., Wieneke, K.: The fine structure of mucosal epithelial cells of a pathological human gall bladder. Anat. Rec.154, 579–616 (1966)

    Article  PubMed  CAS  Google Scholar 

  • Cohn, Z. A.: The structure and function of monocytes and macrophages. Advanc. Immunol.9, 163–214 (1968)

    Article  CAS  Google Scholar 

  • Cookson, F. B.: The origin of foam cells in atherosclerosis. Brit. J. exp. Path.52, 62–69 (1971)

    PubMed  CAS  Google Scholar 

  • Day, A. J.: Lipid metabolism by macrophages and its relationship to atherosclerosis. Advanc. Lipid Res.5, 185–207 (1967)

    CAS  Google Scholar 

  • Dowell, W. C. T.: Die Entwicklung geeigneter Folien für elektronenmikroskopische Präparatträger großen Durchlaßbereiches und ihre Verwendung zur Untersuchung von Kristallen. Optik21, 47–58 (1964)

    Google Scholar 

  • Evett, R. D., Higgins, J. A., Brown, A. L., Jr.: The fine structure of normal mucosa in human gall bladder. Gastroenterology47, 49–60 (1964)

    PubMed  CAS  Google Scholar 

  • Ferner, H.: Über das Epithel der menschlichen Gallenblase. Z. Zellforsch.34, 503–513 (1949)

    PubMed  CAS  Google Scholar 

  • Ganguly, J., Murthy, S. K.: Absorption of cholesterol and vitamin A in rats. In: Meng, H, C. (ed.), Lipid transport, pp. 22–32. Springfield, Illinois: C. C. Thomas Publ. 1964

    Google Scholar 

  • Grosse, H.: Das Problem der Stippchengallenblase. Zwanglose Abhandlungen aus dem Gebiet der normalen und pathologischen Anatomie. Bargmann, W. und Doerr, W. (Hrsg.), Heft 11. Stuttgart: Thieme 1961

    Google Scholar 

  • Grosse, H.: Die Cholelithiasis. Jena: VEB G. Fischer 1966

    Google Scholar 

  • Höra, F., Schulz, H.: Die Ultrastruktur der Stippchengallenblase. Beitr. Path.141, 195–212 (1970)

    Google Scholar 

  • Laitio, M., Nevalainen, T.: Scanning and transmission electron microscope observations on human gallbladder epithelium. I. Adult structure. Z. Anat. Entwickl.-Gesch.136, 319–325 (1972)

    Article  CAS  Google Scholar 

  • Lydon, J. E., Robinson, D. G.: The structure of cholesterylester mesophases revealed by freezefracturing. Biochim. biophys. Acta (Amst.)260, 298–311 (1972)

    CAS  Google Scholar 

  • Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Phys.32, 1637 (1961)

    Google Scholar 

  • Nevalainen, T., Laitio, M.: Ultrastructure of gallbladder with cholesterosis. Virchows Arch. Abt. B10, 237–242(1972)

    CAS  Google Scholar 

  • Parker, F., Ödland, G. F.: Experimental xanthoma. A correlative biochemical, histologic, histochemical, and electron microscopic study. Amer. J. Path.53, 537–565 (1968)

    PubMed  CAS  Google Scholar 

  • Partin, J. C., Schubert, W. K.: Small intestinal mucosa in cholesterol ester storage disease: A light and electron microscope study. Gastroenterology57, 542–558 (1969)

    PubMed  CAS  Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963)

    Article  PubMed  CAS  Google Scholar 

  • Ruska, H., Ruska, C., Meyer-Delpho, W.: Das Gefrierätzbild von Lipidablagerungen in der Aortenintima cholesteringefütterter Kaninchen. Virchows Arch. Abt. B11, 279–283 (1972)

    CAS  Google Scholar 

  • Sterzing, P. R., Napolitano, L. M.: Tissue cholesterol preservation: Factors associated with retention of cholesterol in rat sciatic nerve fixed for electron microscopy. Anat. Rec.173, 485–492 (1972)

    Article  PubMed  CAS  Google Scholar 

  • Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25, 407–408 (1965)

    Article  PubMed  CAS  Google Scholar 

  • Virchow, R.: Über das Epithel der Gallenblase und über einen intermediären Stoffwechsel des Fettes. Virchows Arch. path. Anat.11, 574–578 (1857)

    Google Scholar 

  • Wallraff, J.: Gallengangsystem, Gallenblase und Galle. In: Bargmann, W. (Hrsg.), Handbuch der mikroskopischen Anatomie, Bd. V/4, S. 277–363. Berlin-Heidelberg-New York: Springer 1969

    Google Scholar 

  • Watson, M. L.: Staining of tissue section for electron microscopy with heavy metals. J. biophys. biochem. Cytol.4, 475–478 (1958)

    Article  PubMed  CAS  Google Scholar 

  • Wolf-Heidegger, G., Stäubli, W., Hess, R.: Zur Ultrastruktur und Histochemie der Gallenblasenschleimhaut des Menschen und der Katze. Acta anat. (Basel)62, 606–618 (1965)

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Anatomisches Institut, Abteilung Elektronenmikroskopie, Medizinische Hochschule Hannover, Deutschland

    L. Luciano & C. Wolpers

  2. Kronsforder Allee 3b, D-2400, Lübeck, Bundesrepublik Deutschland

    C. Wolpers

Authors
  1. L. Luciano
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  2. C. Wolpers
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Luciano, L., Wolpers, C. Die Feinstruktur der Gallenblase und der Gallengänge. Virchows Arch. Abt. B Zellpath. 14, 147–158 (1973). https://doi.org/10.1007/BF02889184

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  • DOI: https://doi.org/10.1007/BF02889184

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