Abstract
Plants from 6 potato cultivars uniformly infected with potato virus X (PVX) were exposed to darkness and gradual temperature increases from 37 to 40 C, during a two-week period. This was followed by shoot tip culture to obtain virus free individuals. The procedure was effective in obtaining PVX-free propagation material from 4 of the 6 varieties. Differences were observed in the response of the cultivars to the heat and to thein vitro culture. At least one plant from every cultivar showed repeated negative serological reaction against PVX after the tissue culture regeneration, except Atzimba and Montsama. Tollocan and Murca were the most resistant to heat, developed better duringin vitro culture and resulted in the largest number of PVX-negative plants. Rosita was intermediate, and Juanita did not adapt to heat stress.
Resumen
Se expusieron plantas de papa de 6 cultivares que estaban uniformemente infectadas con virus X de la papa (PVX), a oscuridad e incremento gradual de temperatura de 37 a 40 C, durante un periodo de dos semanas, procediendo posteriormente al cultivo de ápices para obtener individuos libres de virus. El procedimiento fué efectivo en la obtención de material propagativo libre de PVX en 4 de las 6 variedades. Se observaron diferencias en la respuesta de los cultivares al calor y al cultivoin vitro. Por lo menos un individuo mostró repetida reactión serológica negativa contra PVX después de la regeneración en cultivo de tejidos de todas las variedades, excepto Atzimba y Montsama. Tollocan y Murca fueron las más resistentes al calor, las de mejor desarrollo durante el cultivoin vitro y las que resultaron con el mayor número de plantas negativas a PVX. Rosita fué intermedia y Juanita no se adaptó a las condiciones a las que fué expuesta.
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Lozoya-Saldaña, H., Merlin-Lara, O. Thermotherapy and tissue culture for elimination of potato virus X (PVX) in Mexican potato cultivars resistant to late blight. American Potato Journal 61, 735–739 (1984). https://doi.org/10.1007/BF02888976
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DOI: https://doi.org/10.1007/BF02888976