Summary
The present study was designed to utilize the chemiluminescence (CL) method for O2− analysis in ischemia and reperfusion of the whole rat liver. The results indicated that the O2− level was lower after 30 and 60 min ischemia. After 40 min reperfusion, the level of O2− increased obviously, reaching about 8. 3-fold (30 min ischemia) and 9. 0-fold (60 min ischemia) (P<0. 001,P<0. 01) respectively. The results also show that Chinese Traditional Medicines ligustrazine and salvia compound can scavenge O2− effectively.
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Zhang Z J. The Effects of Ligustrazine on the Oxygen Radicals. In: Third Conference on Free Radical Biology. Xiamen, 1990. 134.
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Wei-guo, W., Fa-zu, Q. A new method of superoxide free radical determination in ischemia and reperfusion injury of rat liver. Journal of Tongji Medical University 15, 151–153 (1995). https://doi.org/10.1007/BF02888224
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DOI: https://doi.org/10.1007/BF02888224