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Construction of shuttle expression plasmid and stable expression of foreign gene inmycobacteria andE. Coli

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Summary

By employing the pUC19 as a backbone, the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors.

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Authors and Affiliations

  1. Department of Medical Molecular Biology, Research Center of Experimental Medicine, Tongji Medical University, 430030, Wuhan

    Huangfu Yong-mu, Zhang Da-jun, Cheng Ji-zhong, Qian Min, Liang Ju-qing & Li Dong

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  1. Huangfu Yong-mu
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  2. Zhang Da-jun
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  3. Cheng Ji-zhong
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  4. Qian Min
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  5. Liang Ju-qing
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  6. Li Dong
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Additional information

This work was funded from National Science Foundation of China.

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Yong-mu, H., Da-jun, Z., Ji-zhong, C. et al. Construction of shuttle expression plasmid and stable expression of foreign gene inmycobacteria andE. Coli . Journal of Tongji Medical University 15, 138–142 (1995). https://doi.org/10.1007/BF02888221

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  • DOI: https://doi.org/10.1007/BF02888221

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