Zusammenfassung
Lymphozyten von Müttern und ihren Neugeborenen wurden mit verschiedenen Konzentrationen von Diethylstilböstrol (10-6M, 10-5M und 10-4M) und Mitomycin C (0,003, 0,009 und 0,03 μg/ml) behandelt und zur Bestimmung des Schwesterchromatidaustausches (SCE) kultiviert. Unsere Ergebnisse zeigten, daß die Lymphozyten Neugeborener eine deutlich niedrigere spontane SCE-Rate aufweisen als mütterliche Zellen. Mitomycin C bewirkte einen dosisabhängigen SCE-Anstieg, während Diethylstilböstrol ohne Effekt blieb. Keine Hinweise ergaben sich dafür, daß humane mütterliche Lymphozyten im Vergleich zu den Zellen Neugeborener eine unterschiedliche Sensitivität gegenüber der chemischen SCE-Induktion in vitro besäßen.
Abstract
Lymphocytes of mothers and their newborn infants were treated with varying concentrations of diethylstilbestrol (10−6M, 10−5 M and 10−4M) and mitomycin C (0.003, 0.009 and 0.03 μg/ml), and the incidence of sister chromatid exchange (SCE) was determined. The results showed that the spontaneous SCE rate in the newborn is less than that of their mothers. A dose-related increase in SCEs was oberserved in cells exposed to the alkylating chemical mitomycin C, while increases in SCEs were not noted in cultures exposed to diethylstilbestrol. No different susceptibilities of lymphocytes from mothers and newborns to the chemical induction were observed.
Literatur
Anderson D, Styles JA. The bacterial mutation test. Br J. Cancer 1978; 37: 924–30.
Dunkelberg, H, J. Krames. Sister-chromatid exchange in cultured lymphocytes of ewes and their newborn lambs. Mutation Res 1984; 140: 117–21.
Glatt HR, et al. Diethylstilbestrol and 11 Derivatives. A mutagenicity study with Salmonella typhimurium. Mutation Res 1979; 67: 113–21.
Hartley-Asp B, et al. Diethylstilbestrol induces metaphase arrest and inhibits microtubule assembly Mutation Res 1985:143: 231–35.
Herbst AL, et al. Adenocarcinoma of the vagina. Association of maternal stilbestrol therapy with tumor appearance in young women. New Eng J. Med 1971; 284: 878–81.
Hill A, et al. Increased induction of sister chromatid exchange by diethylstilbestrol in lymphocytes from pregnant and premenopausal women. Cancer Res 1982;42: 893–6.
Lamberti L, et al. Cell kinetics and sister-chromatid-exchange frequency in human lymphocytes. Mutation Res 1983; 120: 193–9.
Lundberg MS, Livingston GK. Sister-chromatid exchange frequency in lymphocytes of smoking and nonsmoking mothers and their newborn infants. Mutation Res 1983; 121: 241–6.
Mehnert K, et al. Effect of diethylstilbestrol on the frequencies of sister chromatid exchange in vitro and in vivo. Cancer Res 1985; 45: 3626–30.
Musilova J, et al. Induction of sister chromatid exchanges by Mitomycin C in lymphocytes of young and old human donors. Gerontology 1984; 30: 365–70.
Schneider E, et al. The effect of aging on sister chromatid exchange. Mechanisms of Ageing and Development 1979; 9: 303–11.
Seshadri R, et al. Sister chromatid exchange (SCE) analysis in mothers exposed to DNA-damaging agents and their newborn infants. Mutation Res 1982; 97: 139–46.
Styles JA. Mammalian cell transformation in vitro. Brit. J. Cancer 1978; 37: 931–36.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Xue-min, C., Schmidt, B., Dunkelberg, H. et al. Zur Induktion von Schwesterchromatidaustausch durch Diethylstilböstrol und Mitomycin C bei Lymphocyten von schwangeren Frauen und deren Neugeborenen. Journal of Tongji Medical University 7, 191–194 (1987). https://doi.org/10.1007/BF02888218
Issue Date:
DOI: https://doi.org/10.1007/BF02888218