Zusammenfassung
Das Hornhautendothel zeigte bei der Gewebelappenkultur membranartigen Wachstumscharakter. Die kultivierten Zellen waren vieleckig. Im menschlichen Hornhautendothel, einschichtig, wurden amitotische Erscheinungen beobachtet. Das gezüchtete Endothel wies unter dem Elektronenrastermikroskop mehr oberflächliche Mikrovilli auf als das Endothel in vivo. Mit dem Transmissionselektronenmikroskop wurden zwischen den Endothelzellen große Desmosome, und in den Zellen viele gebündelte, gelockte faserige Strukturen, die retikulär um den Kern herum lagen, demonstriert. Die Zellen waren reich an Organellen. Über die Regenerationsfähigkeit des Hornhautendothels und einige biologische Charakteristika des gezüchteten Endothels wird diskutiert. Wir hoben hervor, daß sich die Zellen des menschlichen Hornhautendothels unter bestimmten Bedingungen vermehren können, aber die Art und Weise der Vermehrung muß noch eingehend erforscht werden.
Abstract
In in-vitro culture, we have found that corneal endothelial cells exhibited membranous growth character in tissue culture. The cells were polygonal in shape. When human corneal endothelium grew in single layer, we found the phenomenon of amitosis. Scanning electronmicroscopy showed the microvilli of endothelial cells in vitro were more than in vivo. Transmission electronmicroscopy demonstrated a lot of intercellular desmosome and many fibrillar structures accumulated into bundles, curly-hair-like in shape and distributed reticularly around the nucleus. The cells were rich in organelles. We have also discussed the regenerative ability and some biological characteristics of corneal endothelium. It may be suggested that human corneal endothelia exhibit the proliferative ability in some condition. However, their proliferative form remains to be further studied.
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Hong, Y., Luo, Z. & Xiao-kang, Z. Das Wachstum und die morphologischen Charakteristika des Hornhautendothels vom Menschen und vom Kaninchen in der Kultur. Journal of Tongji Medical University 11, 116–122 (1991). https://doi.org/10.1007/BF02888100
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DOI: https://doi.org/10.1007/BF02888100