Summary
In order to provide a new method for treating proliferative vitreoretinopathy (PVR), the effects of anti-proliferation and apoptosis induction of inhibitors of telomerase and heat shock protein 90 (Hsp90) on the cultured retinal pigment epithelial (RPE) cells were investigated. The rate of apoptosis cells was measured by using TUNEL on the cultured RPE cells, the co-cultured RPE cells with inhibitor of telomerase (camptothecin) or the co-cultured RPE cells with inhibitor of Hsp90 (geldanamycin). The cell proliferation status was measured in the above three groups by using MTT method. The rate of apoptosis in the RPE cells co-cultured with camptothecin or geldanamycin was increased remarkably (P< 0.05). MTT showed the rate of growth inhibition was 8.4 %, 32.3 % and 72.3 % at the concentrations of camptothecin 1 μmol/L, 5 μmol/L, 10 μmol/L, respectively, and 6.5 %., 30.9 %, 71.9 % at the concentrations of geldanamycin 1 μmol/L, 5 μmol/L, 10 μmol/ L, respectively. It was concluded that telomerase and Hsp90 can promote the proliferation of the cultured RPE cells, while the inhibitor of them can induce apoptosis and inhibit the growth of the RPE cells.
Similar content being viewed by others
References
1999, 23:286
Flood M T, Goruras P, Kjeldbyc Het al. Growth characteristics and ultra-structure of human retinal pigment epithelialin vitro. Invest Ophthalmol Vis Sci, 1980, 19: 1309
1995.
1996.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. J Immunol Method, 1983, 65:55
Planchon S M, Wuerzberger S, Frydman Bet al. β-Lapachone-mediated apoptosis in human promyelocytic leukemia (HL-60) and human prostate cancer cells: a p53-independent response. Cancer Research, 1995, 55 (17):3706
Arno H, Peter E, Klaus Het al. The topoisomerase I inhibitors, camptothcin and β-Lapachone induce apoptosis of human retinal epithelial cells. Exp Eye Res, 1998, 67: 525
Zhang Z, Liong E C, Lau T Yet al. Induction of apoptosis by hexamethylene bisacatamide is p53-dependent associated with telomerase activity but not with terminal differentiaton. Int J Oncol, 2000, 16:887
Stebbins C E, Russo A A, Schneider Cet al. Crystal structure of an Hsp90-Geldanamycin complex: Targeting of a protein chaperone by an antitumor agent. Cell, 1997, 89(2):239
Holt S E, Aisner D L, Baur Jet al. Functional requirement of P23 and Hsp90 in telomerase complex. Genes Dev, 1999, 13:817
Yoon H S, Rho S H, Jeong J Het al. Genistein produce reduction in growth and induce apoptosis of rat RPE-J cells. Curr Eye Rws, 2000, 20:215
Hueber A, Esser P, Heimann Ket al. The topoisomerse I inhibitors, camptothecin and beta-lapachone, induce apoptosis of human retinal pigment epithelial cells. Exp Eye Res, 1998, 67(5):525
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yi, X., Shuiqing, Z. The anti-proliferative effect of inhibitor of telomerase on cultured retinal pigment epithelial cells. Current Medical Science 21, 174–176 (2001). https://doi.org/10.1007/BF02888090
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02888090