Skip to main content
SpringerLink
Log in

The influence of phorbol ester on the effect of tamoxifen in breast cancer cells

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

To investigate the correlation between the activity of kinases in the growth factor signal transduction pathway and the development of resistance of breast cancer to tamoxifen, reporter gene regulated by the regulating fragment of CCD1 was transfected into the MCF-7 cells, and the influence of tamoxifen on the reporter gene expression was examined under different conditions of TPA treatment. Our results showed that the reporter gene expression was inhibited by tamoxifen and promoted by TPA. Furthermore, tamoxifen exerts an agonist effect on the reporter gene expression when the cells was treated by TPA previously for 12 h. It is concluded that TPA could induce estrogen-like effect of tamoxifen on estrogen receptor positive breast cancer cells and it may be one of the mechanisms responsible for the development of tamoxifen resistance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mueller H, Loop P. Liu Ret al. Differential signal transduction of epidermal-growth factor receptors in hormonedependent and hormone-independent human breast cancer cells. Eur J Biochem, 1994, 221:631

    Article  CAS  PubMed  Google Scholar 

  2. Horwitz K B, McGuire W L. Nuclear mechanisms of estrogen action. Effects of estradiol and anti-estrogens on estrogen receptors and nuclear receptor processing. J Biol Chem, 1978, 253:8185

    Article  CAS  PubMed  Google Scholar 

  3. de Wet J R, Wood K V, Deluca Met al. Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol, 1987, 7:725

    PubMed  PubMed Central  Google Scholar 

  4. Valette A, Gas N, Jozan Set al. Influence of 12-O-tetradecanoylphorbol-13-acetate on proliferation and maturation of human breast carcinoma cells (MCF-7): relationship to cell cycle events. Cancer Res, 1987, 47:1615

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan

    Cai Kailin M. D., Ph. D., Chen Daoda, Wang Guobin, Lu Xiaoming, Zhang Jinhui & Tian Yuan

Authors
  1. Cai Kailin M. D., Ph. D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chen Daoda
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wang Guobin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lu Xiaoming
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhang Jinhui
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tian Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This project was supported by a grant of the National Natural Sciences Foundation of China (No. 39770724).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kailin, C., Daoda, C., Guobin, W. et al. The influence of phorbol ester on the effect of tamoxifen in breast cancer cells. Current Medical Science 21, 138–139 (2001). https://doi.org/10.1007/BF02888078

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02888078

Key words

Search

Navigation