Summary
To study the osteogenic potential of cultured bone marrow stromal cells (BMSCs) transfected with transforming growth factor β1 (TGF-β1) genein vitro, cultured BMSCs were transfected with the complexes of pcDNA3-TGF-β1 and Lipofectamine Reagentin vitro. The cell proliferation was detected by MTT method and the morphological features of transfected BMSCs was observed. ALP stains and PNP method were used to measure ALP activity. In addition, the collagen type I propeptides and mineralized matrixes were examined by immunohistochemical staining and tetracycline fluorescence labeling respectively. The morphological and biological characters of the transfected BMSCs were similar to those of osteoblasts and the cell proliferation was promoted. The cell layer displayed strong positive reaction for ALP stains and immunohistochemical staining. ALP activity and collagen type I expression increased remarkably after transfection. Mineralized matrixes formed earlier and more in transfected BMSCs as compared with control group. It is concluded that transfecting with TGF-β1 gene could promote the osteogenic potential of cultured BMSCs.
Similar content being viewed by others
References
Herbertson A, Aubin J E. Cell sorting enriches osteogenic populations in rat bone marrow stroraal cell cultures. Bone, 1997, 21(6):491
1999, 20(4): 201
β1 2000, 17(4):344
Rath M, Niendorf A, Reblin Tet al. Detection and quantification of lipoprotein in the arteral wall of 107 coronary bypass patients. Arteriosclerosis, 1989, 9(3): 579
Kassem M, Risteli L, Mosekilde Let al. Formation of osteoblastlike cells from mononuclear bone marrow cultures. APMIS, 1991, 99(3):269
1997,11(4):238
Goshima J, Goldbery V M, Caplam A Iet al. The osteogenic potential of culture-expanded rat marrow mesenchymal cells assayedin vivo in calcium phosphate ceramic blocks. Clin Orthop, 1991, 262:298
Cornell C N, Lane J. Newest factors in facture healing. Clin Orthop, 1992, 277:297
Bonewald L F, Mundy G R. Role of transforming growth factor beta in bone remodeling. Clin Orthop, 1990, 250:261
Locklin R M, Williamson M C, Beresfird J Netal. In vitro effects of growth factors and dexamethasone on rat marrow stromal cells. Clin Orthop, 1995, 313:27
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chengqing, Y., Qixin, Z., Xiaodong, G. et al. Osteogenic potential of cultured bone marrow stromal cells transfected with transforming growth factor β1 genein vitro. Current Medical Science 21, 130–133 (2001). https://doi.org/10.1007/BF02888076
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02888076