Summary
Regulation of the adhesion molecules expression by cytokine in vascular endothelial cells was investigated. Human umbilical vein endothelial cells (HUVEC) were stimulated with cytokines, TNF-α (1–250 U/ml) or IL-1ß (0. 1–50 U/ml) for 24 h. HUVEC were also cultured with cytokines, TNF-α (100 U/ml) or IL-1ß (10 U/ml), for 4–72 h, cell surface expression of adhesion molecules (ICAM-1 and VCAM-1) were detected and quantitated by immunocytochemical methods and computerized imaging analysis technique. Adhesion molecules expression were up-regulated by TNF-α, IL-1ß in a concentration- and time-dependent manner. Some significant differences were observed between the effects of cytokines on the ICAM-1 and on VCAM-1 expression. Cytokines might directly induce the expression of ICAM-1 and VCAM-1 in vascular endothelial cells. Our observations indicate differential functions of the two adhesion molecules during the evolution of inflammatory responses in stroke.
Similar content being viewed by others
References
Stanimirovic D B, Wong J, Shapiro Aet al. Increase in surface expression of ICAM-1, VCAM-1 and E-selectin in human cerebromicrovascular endothelial cells subjected to ischemia-like insults. Acta Neurochir, 1997(suppl), 70: 12
TNF ICAM, 1999, 28(6): 489
Bitsch A, Klene W, Murtada Let al. A longitudinal prospective study of soluble adhesion molecules in acute stroke. Stroke, 1998, 29: 2129
Wong D, Dorovini K. Expression of vascular cell adhesion molecule-1 (VCAM-1) by human brain microvessel endothelial cells in primary culture. Microvascular Research, 1995, 49: 325
HUVEC EC 1998, 19(4): 394
Christian W, Emil N, Wolfgang Eet al. Inhibitors of protein tyrosine kinase suppress TNF-stimulated induction of endothelial cell adhesion molecules. The journal of immunology, 1995, 155: 445
Barone F C, Arvin B, White B Set al. Tumor Necrosis Factor-α mediator of focal ischemic brain injury. Stroke, 1997, 28: 1233
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Honghui, C., Changqin, L., Shenggang, S. et al. Cytokine-induced cell surface expression of adhesion molecules in vascular endothelial cellsIn vitro. Current Medical Science 21, 68–71 (2001). https://doi.org/10.1007/BF02888042
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02888042