Construction and expression of eukaryotic expressing vector pCH510 of polypeptide CH50 and its chemotaxis and antitumor function byin vivo transfection

Summary

To construct an eukaryotic expressing vector that expresses CH50, a recombinant Cell I-Hep I bifunctional-domain polypeptide of human fibronectin, and to investigate the chemotaxis to immune cells and the inhibitory effect on the growth of tumor by the expression of the plasmidin vivo, the plasmid was constructed by DNA recombination. Gene transfection was performedin vitro andin vivo. The expressed product was identified by Western blot. The chemotaxis after gene transfectionin vivo was observed by histotomy and staining of muscle tissues. The inhibition of gene transfection on solid tumor was observed in mice. The results showed that plasmid pCH510 was constructed by the recombination of the 5′-terminal noncoding region and signal peptide coding region of human fibronectin cDNA and cDNA fragment coding CH50 polypeptide with a 3′-terminal noncoding region of human FN cDNA, and the insertion of the recombinated fragment into plasmid pcDNA3. 1. After transfection with plasmid pCH510, NIH3T3 cells could produce CH50 polypeptide. The transfection of plasmid pCH510 by the injection in muscle of mouse could produce the effects of chemotaxis on immune cells and the inhibition on the growth of solid tumor. It is concluded that plasmid pCH510 can express in cells andin vivo in mouse. The expression of the plasmidin vivo has a chemotactic effect on immune cells and can inhibit the growth of solid tumor.