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Effect of concurrent use of rh-IL-3 and rh-GM-CSF on apoptosis of HL-60 cells induced by Ara-C

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Summary

The myeloid leukemic cell line HL-60 was studied by using DNA gel electrophoresis, flow cytomery, McAb C-myc, MeAb Bcl-2 and CFU-L. From zero to 36 h, the apoptosis rates of 8 different phases and other indexes were observed. The results showed that with the prolonged time of drug incubation, apoptosis of HL-60 cells increased progressively. This effect can be enhanced obviously by rh-IL-3 and rh-GM-CSF. At the same time, the killed rate of leukemic cells by Ara-C induction was increased. C-myc expression was decreased and Bcl-2 expression did not display apparent change. Interestingly, the normal hemopoietic cells were not affected by these two kinds of cytokine. The theoretical basis was provided for concurrent use of rh-IL-3, rh-GM-CSF and cytotoxic drugs whose purpose is to elevate remission rate during the phase of induced remission of leukemia.

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References

  1. 1995,3:15

  2. Sachs L, Lotem J. Control of programmed cell death in normal and leukemic cells: new implications for therapy. Blood, 1993, 82:15

    Article  CAS  PubMed  Google Scholar 

  3. 1988,3:89

  4. Binder C, Hiddemann W. Programmed cell death-many questions still to be answered. Ann Hematol, 1994, 69:45

    Article  CAS  PubMed  Google Scholar 

  5. Bhalla K, Tang C, Ibrado A Met al. Granulocyte-macrophage colony-stimulating factor/interleukin 3 fusion proteins (PIXY 321) enhances high-dose Ara-C-induced programmed cell death or apoptosis in human myeloid leukemia cells. Blood, 1992, 30:2883

    Article  Google Scholar 

  6. Bhalla K, Tourkina E, Huang Yet al. Effect of hemopoietic growth factors G-CSF and PIXY 321 on the activity of high dose Ara-C in human myeloid leukemia cells. Leuk lymphoma, 1993, 10(suppl):123

    Article  PubMed  Google Scholar 

  7. Yamada K, Furusawa S, Saito Ket al. Concurreny use of granulocyte colony-stimulating factor with low-dose cytosine arabinoside and aclarubicin for previously treated acute myelogenous leukemia: a pilot study. Leukemia, 1995, 9: 10

    CAS  PubMed  Google Scholar 

  8. Sen S, Programmed cell death: concept, mechanism and control. Biol Rev Camb Philos Soc, 1992, 67:287

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institute of Hematology, Xiehe Hospital, Tongji Medical University, 430022, Wuhan

    Chen Yan, Zhou Jianfeng, Li Chongyu, Wang Bianming & Li Huiyu

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  1. Chen Yan
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  2. Zhou Jianfeng
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  3. Li Chongyu
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  4. Wang Bianming
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  5. Li Huiyu
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Yan, C., Jianfeng, Z., Chongyu, L. et al. Effect of concurrent use of rh-IL-3 and rh-GM-CSF on apoptosis of HL-60 cells induced by Ara-C. Current Medical Science 17, 13–17 (1997). https://doi.org/10.1007/BF02887994

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  • DOI: https://doi.org/10.1007/BF02887994

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