Summary
The interference of Cyclosporin A (CyA) with peripheral lymphocytes DNA synthesis was investigated. The results showed that the drug could strongly inhibit T cell proliferation in response to PHA stimulation if added at the start or at the early phase of the process, and it failed to do so if added 48 h later when most cells had already started to proliferate. Without PHA, no obvious differences were detected between normal control and CyAtreated cells. A comparison was made between T cell DNA synthesis of peripheral blood lymphocytes of healthy volunteers and renal transplant patients. It was found that T-cell DNA synthesis of patients was significantly lower than that of the healthy controls. From the above it may be suggested that CyA activity on T cell proliferation is similar in vivo and in vitro.
Similar content being viewed by others
References
Woo J, et al. The influence of FK-506 and low-concentration cyclosporin on human lymphocyte activation antigen expression and blastogenesis: a flow cytometric analysis. J Immunol 1990;31: 297
Hess AD. Effect of interleukin 2 on the immunosuppresive action of cyclosporin. Transplantation 1985:39:62–8.
Kermani-Arab V, et al. Mediation of the antiproliferative effect of cyclosporin on human lymphocytes by blockade of 1L-2 biosynthesis. Transplantation 1985:39:439
Landegren U, Wigzell H. Cyclosporin A inhibits a discrete step in T-lymphocyte stimulation. Scand J Immunol 1985;22: 284–97.
1989;5(2):100–2.
Buurman WA, et al. Cyclosporin A inhibits IL 2-driven proliferation of human alloactivated T cells. J Immunol 1986;136:4035–9.
Koponen M, et al. The effects of cyclosporins on the cell cycle of T-lymphoid cell lines. Exp Cell Res 1982:140: 237–50.
Gunter KC, et al. CyA-mediated inhibition of mitogehinduced gene transcription is specific for the mitogenic stimulus and ceil type. J Immunol 1989:142:3286–91.
Krorske M, et al. Cyclosporin A inhibits T cell growth factor gene expression at the level of mRNA transcription. Proc Natl Acad Sci USA 1984:81:5214–20.
1990:15:243–8.
1985;1(2):5–7.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ming, L., Han, Z., Jing, Y. et al. Inhibitory effect of cyclosporin A on T lymphocyte DNA synthesis. Journal of Tongji Medical University 12, 39–42 (1992). https://doi.org/10.1007/BF02887757
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02887757